SALINOMYCIN INDUCED APOPTOSIS IN HUMAN PROSTATE CANCER CELLS

Virtually all prostate cancer deaths occur due to obtaining the castration-resistant phenotype after prostate cancer cells escaped from apoptosis and/or growth suppression initially induced by androgen receptor blockade. TNF-related apoptosis-inducing ligand (TRAIL) was an attractive cancer therapeu[r]

Aldehyde dehydrogenase 1A3 (ALDH1A3) has been implicated in the survival and proliferation of prostate cancer cells.

Some studies reported that intracellular Ca 2+ flux also mediates multiple cellular signaling cascades of survival and apoptosis. Release of Ca 2+ from ER and high Ca 2+ concentration in cytosol trigger cell death in human cancer cell lines [44]. Our results indica[r]

Metastatic melanoma is an aggressive form of skin cancer that evades various anti-cancer treatments including surgery, radio-,immuno- and chemo-therapy. TRAIL-induced apoptosis is a desirable method to treat melanoma since, unlike other treatments, it does not harm non-cancerous cells.

64 CHAPTER 3 JNK-dependent phosphorylation of c-Jun on Ser-63 mediates NO-inducible apoptosis in human SH-Sy5y neuroblastoma cells.... 65 3.1 NO induces concentration-dependent apoptosis[r]

Prostate cancer is the most commonly diagnosed malignancy among men. The Discovery of new agents for the treatment of prostate cancer is urgently needed. Compound WZ35, a novel analog of the natural product curcumin, exhibited good anti-prostate cancer activity, with an IC50 of 2.2 μM in PC-3 cells.

In a cytotoxicity screen in serum-free medium, Guttiferone F showed strong growth inhibitory effect against prostate cancer cells. Methods: Prostate cancer cells LNCaP and PC3 were treated with Guttiferone F in serum depleted medium. Sub-G1 phase distributions were estimated with flow cytometry. Mit[r]

incubator at 37 o C. Since DNA fragmentation is a hallmark of apoptotic cell death, the virus infected cells were scraped at regular time interval and harvested after centrifugation at 3,000 rpm for 10 min [5,10]. The DNA was extracted by the phenol-chroloform method [11]. The extracted DNA w[r]

I found that accumulation of misfolded N-CoR in the ER stimulates UPR-induced apoptosis in non-APL cells, while in APL cells, this apoptotic response is neutralised through the processin[r]

Genistein has been known to inhibit proliferation and induce apoptosis in several kinds of cancer cells. While knowledge of genistein in regulating epithelial mesenchymal transition (EMT) of colon cancer cells is unknown.

Jan Roger Olsen 1,6* , Waqas Azeem 1,2 † , Margrete Reime Hellem 1 † , Kristo Marvyin 1 , Yaping Hua 1 , Yi Qu 1,3 , Lisha Li 4 , Biaoyang Lin 4,5 , XI-Song Ke 1 , Anne Margrete Øyan 1 and Karl-Henning Kalland 1,2,3,6*
Abstract
Background: Expression of the androgen receptor (AR) is associated w[r]

ROS production was measured using fluorescent probes DHE, and MitoSOX. Cells seeded onto 6-well plates were first stained with either DHE (10 μ M) or MitoSOX (5 μ M) in Hanks ’ balanced salt solution (HBSS) for 30 min (15 min in case of MitoSOX) at 37°C. After washing away unbou[r]

Apoptosis(programmed cell death) is a common cellular response to virus infection [4]. Cell culture studies have established that many common respiratory viruses can induce apoptosis in epithelial cells [5,6]. Recent work has demonstrated that viral infections can activat[r]

Radiosensitisation caused by titanium dioxide nanoparticles (TiO2-NPs) is investigated using phantoms (PRESAGE® dosimeters) and in vitro using two types of cell lines, cultured human keratinocyte (HaCaT) and prostate cancer (DU145) cells.

When curcumin was applied topically to the dorsal skin of female ICR mice (a model initially developed at the Institute of Cancer Research, Fox Chase Cancer Center), it prevented the PMA-induced activation of both Nf- κ b and Ap1 ( REF. 25 ). The inhibition of Nf- κ b was accomp[r]

Advanced prostate cancer commonly metastasizes to bone leading to osteoblastic and osteolytic lesions. Although an osteolytic component governed by activation of bone resorbing osteoclasts is prominent in prostate cancer metastasis, the molecular mechanisms of prostate cancer-induced osteoclastogene[r]

Schisandra chinensis has been shown to suppress the TPA-induced skin tumor formation and
prostate carcinogenesis [1, 2]. It also aids to protect liver from injury, stimulates liver regeneration and
reduces inflammation. Panax ginseng and Ziziphus jujube provide several pharmacolog[r]

termed COS-7-OPH for further experiments and were maintained with 1 mg/ml G418.
Glutathione (GSH) depletion assay
A volume of 180 μ l of a freshly prepared solution containing 65 μ M GSH and 160 μ M of prodrug (or a mixture of 160 μ M R-NPAA and 160 μ M S-ANAA for inhibition assay) in 50[r]

Overexpression of FHL1C induces apoptosis of T-ALL cells To examine the role of FHL1C in T-ALL, we transiently overexpressed FHL1C in Jurkat cells, a human T-ALL cell line bearing Notch1[r]

Previous studies showed sanguinarine induced apoptosis in CRC cells but did not define the underlying mechanisms. The purpose of this work was to determine the in vivo and in vitro effects of sanguinarine on CRC tumors and to elucidate the mechanism in regulating the intrinsic apoptosis.