cloning, expression and characterization of PTPA, a protein thatactivates the tyrosylphosphatase activity of protein phosphatase2A. J. Biol. Chem. 269, 15668–15675.35. Harlow, I. & Lane, D. (1988) Antibodies, a Laboratory Manual.Cold Spring Harbor Laboratory Press[r]
35. Russo JJ, Bohenzky RA, Chien MC, Chen J, Yan M, Maddalena D, ParryJP, Peruzzi D, Edelman IS, Chang Y, Moore PS: Nucleotidesequence of the Kaposi sarcoma-associated herpesvirus(HHV8). Proc Natl Acad Sci U S A 1996, 93(25):14862-14867.36. Rovnak J, Quackenbush SL, Reyes RA, Baines JD, Parri[r]
reporter expression was stimulated by PMA but notforskolin. The highest response was detected when forskolinand PMA were used together (Fig. 4). In this case theexpression level of the reporter gene was similar to theexpression induced by CRF. Thus, CRF induced the samelevel of respons[r]
permits collection of fetal blood for lymphocyte culture and analysis. In combination with in vitro fertilization (IVF) techniques, it is even possible to perform genetic diagnoses in a single cell removed from the four- to eight-cell embryo or to analyze the first polar body from an o[r]
prokaryotes to Giardia lamblia and Entamoeba histolytica.Eukaryot. Cell 1, 181–190.45. Zhao, G. & Winkler, M.E. (1996) A novel alpha-ketoglutaratereductase activity of the serA-encoded 3-phosphoglycerate dehy-drogenase of Escherichia coli K-12 and its possi[r]
cyclization of squalene and oxidosqualene to sterols andtriterpenes. Chem. Rev. 93, 2189–2206.3. Kushiro, T., Shibuya, M. & Ebizuka, Y. (1998) b-amyrin synthase.Cloning of oxidosqualene cyclase that catalyzes the formation ofthe most popular triterpene among higher plant[r]
murine forms of the mucin. MUC16 is both expressed onthe cell surface and shed from the cell in soluble forms.Muc16, on the other hand, is detected primarily in thespent media and in the intracellular environment. Thisobservation indicates that the Muc16 is either rapidlycleaved[r]
ÔEUROCE LLWALLÕ to L.P., and FIR ST 2001 and 20 02 grant toL.P. C. Carotti was the recipient of a fellowship financed by the sameEU project.References1. Klis, F.M. (1994) Review: cell wall assembly in yeast. Yeast 10,851–869.2. Popolo, L. & Vai, M. (1999) The Gas1 glycopr[r]
.. .DETECTION OF BIOLOGICALLY RELEVANT ANIONS BY FLUORESCENCE AND NIR MOLECULAR PROBES QUEK YI LING (B Sc.(Hons.), National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF. .. Results and discussion .140 6.3.1 Synthesis of complexes and 140 6.3.2 Spectroscopic characterizatio[r]
Mol. Endocrinol. 14, 183–193.35. Wang, W. & Gershengorn, M.C. (1999) Rat TRH receptor type 2exhibits higher basal signaling activity than TRH receptor type 1.Endocrinology 140, 4916–4919.36. Edwards, J.B., Delort, J. & Mallet, J. (1991) Oligodeoxy-ribonucleotide ligation to single-st[r]
σbr and therefore does not present a limitation for this parameter. This is not the first time that changes in the backscattering pattern obtained from a simple configuration allows for the characterization of some geometrical or material changes produced in the scattering objec[r]
tude difference is about to jump again, in the era of metagenomics, as the analyses ofcommunities of largely unculturable organisms in their natural states come to dom-inate sequence production. The J. Craig Venter Institute’s Sargasso Sea experimentand other early metagenomics experim[r]
fractions of colon, breast, and hepatocellular cancers. Most recently, this approach has been applied to an unbiased set of genes including about two-thirds of all those known to encode proteins. Hundreds of genes not previously implicated in cancers were shown to[r]
required for an active intein. The remaining two extra seg-ments (88 and 121 aa at the position 'i1' and 'i2', respec-tively) did not exhibit any significant similarity to knownprotein sequences. The biological properties of thosethree Mimivirus specific inserts remain to bechar[r]