PURIFICATION AND CHARACTERIZATION OF CARBON MONOXIDE DEHYDROGENASE FROM THE AEROBIC HYPERTHERMOPHILI...

that in bacteria.ACKNOWLEDGEMENTSThis work was performed as part of the STA fellowship programsupported by the Japan Science and Technology C orporation.REFERENCES1. Nelson, K.H., Paulsen, I.T., Heidelberg, J.F. & Fraser, C.M.(2000) Sta tus of genome projec[r]

23. Pott, A.S. & D ahl, C. (1998) Sirohaem s ulfite reductase and o therproteins encoded by g e nes at th e dsr locus of Chrom atium vinosumare involved in the oxidation of intracellular sulfur. M icrobiology144, 1881–1894.24. Rossi, M., Pollock, W.B., Reij, M.W.,[r]

ripples, oriented either along [001] or [1–10] directions,mounds, and unexpected rhomboidal pyramids (RP) [17,18]. The latter nanostructures are particularly interesting ascandidate model systems for testing catalytic reactivity,since they are endowed with steep facets running alongnon[r]

37 °C for 16 h. A portion of the sample was diluted in 5%(v ⁄ v) formic acid and the peptides separated using an Ulti-Mate nanoLC (LC Packings, Amsterdam, the Netherlands)equipped with a PepMap C18 trap and column. The eluantwas sprayed into a Q-Star[r]

of the recombinant enzymeWe initially attempted to express the PDH1 gene usingthe plasmid pPDH1, but no functional product couldbe obtained. We therefore introduced the gene intopET11a, and were then able to successfully express itand then isolate it using the