polymerase (Promega, Madison, WI, USA).
In the case
of analysis
of the sequence specificity
of the myristoylation motif, template DNAs for
in vitro transcription were pre- pared from the Myr–AGG1-3X6A, Myr–AGG1-3X6S, Myr–AGG1-3X6A7K
and Myr–AGG1-3X6S7K vect[r]