OTHER METHODS TO STUDY PROTEIN BINDING

For many metalloflavoproteins, most of the conventional apoflavoprotein preparation procedures cause extensive denaturation.A likely explanation for this is that deflavin- ation and reconstitution of flavoproteins is difficult if the quaternary structure is more complex, i.e. when the protein cont[r]

Discussion
Vitamin K-dependent proteins interact through their characteristic Gla domains with phospholipid mem- brane surfaces. The Gla domains exhibit appreciable amino acid sequence similarity. When Ca 2+ is bound, the Gla domain folds into a conserved x -loop exposing surface-orientated hydr[r]

In an effort to expand on the potential protein inter- action map of the anti-cancer drug target MDM2, we previously utilized peptide aptamer libraries to identify novel MDM2-binding proteins [21]. This biochemical approach for expansion of the ‘interactome’ of a target r[r]

Abstract
HIV-1 transcription is tightly regulated: silent in long-term latency and highly active in acutely- infected cells. Transcription is activated by the viral protein Tat, which recruits the elongation factor P-TEFb by binding the TAR sequence present in nascent HIV-1 RNAs. In[r]

Protein-protein interactions (PPIs) are critical for many biological processes. It is therefore important to develop accurate high-throughput methods for identifying PPI to better understand protein function, disease occurrence, and therapy design.

Interestingly, even the conservative replacement of Rad51-Tyr232 by tryptophan affects the dsDNA bind- ing, indicating direct contact between Tyr232 and DNA. Aromatic residues, such as Tyr, Phe, and Trp, can stack with the base moieties of ssDNA for the inter- action. Such contacts have been observe[r]

Prediction of DNA-binding residue is important for understanding the protein-DNA recognition mechanism. Many computational methods have been proposed for the prediction, but most of them do not consider the relationships of evolutionary information between residues.

The mAb VN04-2 was raised against the HA of A/Vietnam/ 1203/04, therefore to select the HAs to be used in this study, we aligned all the HA sequences from H5N1 viruses isolated throughout 2005 and 2006, that were deposited into the Influenza Virus Resource and was maintained by[r]

unaffected even by the highest concentration of protein S used (240 nM, results not shown). (b) In the second analysis, free C4BP and C4BP–protein S complexes were immobilized and the binding of MoAb 15 tested (Moab 44 did not work in this assay system as described above). MoA[r]

Flow of genetic information. Multiple extracellular signals activate intracellular signal cascades that result in altered regulation of gene expression through the interaction of transcription factors with regulatory regions of genes. RNA polymerase transcribes DNA[r]

in the process of fitting the diaphragm and cervical caps and in teaching a woman how to use it correctly, backed by an appro- priate leaflet.
With each of these products, the single most important thing the woman must learn is the vital regular secondary check, after placing it, that sh[r]

band (Fig. 6B). In a reverse experiment, we immuno- precipitated Mpp3 with CPH8 and tested for coimmu- noprecipitation of Mpp4. CPH8 preimmune serum was used as a control. Whereas we could detect Mpp5 in the anti-Mpp3 immunoprecipitate (Fig. 5B), we could not detect Mpp4, although it was readily ide[r]

It seems likely that potential data based on our data and that of others have accuracies of 3–5 mV. Even taking the accuracy into consideration, redox poten- tials of Ec DosH proteins have an apparent hysteresis in the data, being different between the reductive and oxidative potentials (Table 5).[r]

(b) Non-legal enforcement mechanisms
Although there are currently relatively few formal legal mechanisms which non- state actors can use to enforce supranational regulatory norms (with the excep- tion of EU law), there has been considerable scope for private actors to participate in[r]

Drug-protein binding may play a role in the thermal energetics of protein denaturation and could lead to the determination of its equilibrium dissociation parameter. The aim of this study was to assess the energetics of a drug that was bound to human serum albumin (HSA) during thermal denaturation.[r]

Decreased dietary intake of protein may lead to mild to moderate hypoproliferative anemia; this form of anemia may be prevalent in the elderly. The anemia can be more severe in patients with a greater degree of starvation. In marasmus, where patients[r]

The Arabidopsis SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) transcription factor SPL7 reprograms cellular gene expression to adapt plant growth and cellular metabolism to copper (Cu) limited culture conditions.

The aim of this study was to evaluate the role of family history of cancer and personal history of other medical conditions in the aetiology of the oral cavity cancer in France. Methods: We used data from 689 cases of oral cavity squamous cell carcinoma and 3481 controls included in a population-bas[r]

Protein remote homology detection plays a vital role in studies of protein structures and functions. Almost all of the traditional machine leaning methods require fixed length features to represent the protein sequences.

Frog p26olf is a novel S100-like Ca 2 1 -binding protein found in olfactory cilia. It consists of two S100-like domains aligned sequentially, and has a total of four Ca 2 1 -binding sites (known as EF-hands). In this study, to elucidate the mechanism of Ca 2 1 -[r]