IDENTIFICATION OF POTENTIAL PROTEIN BINDING SITES IN 4E

BioMed CentralPage 1 of 10(page number not for citation purposes)Virology JournalOpen AccessResearchThe SARS Coronavirus S Glycoprotein Receptor Binding Domain: Fine Mapping and Functional CharacterizationSamitabh Chakraborti, Ponraj Prabakaran, Xiaodong Xiao and Dimiter S Dimitrov*Add[r]

separated from myc-His6-TN by absorption of the latter onNi-NTA-sepharose. The run through fraction was subjectedto S-Sepharose, which enabled TN purification. Two intensebands each with a relative molecular mass of approximately20 kDa were visualized on SDS/PAGE (Fig. 1). Thesebands co[r]

2ϩ, and two Kϩions. See Figure 31.2a for the location of this site within the ATPase domain.Carbon atoms are gray; oxygen, red; nitrogen, blue; and phosphorus, yellow. P143 sits at the centerof an H-bonded network of residues, which includes K70, Y145, F146, R151, and E171. (b–d) Theme[r]

enzymes associated with energy metabolism during the early post mortem period in longissi-mus thoracis bovine muscle analyzed by proteomics. Journal of Proteome Research, 5, 1763–1769.Kandarian, S. C. & Jackman, R. W. (2006). Intracellular signaling during skeletal muscle atrop[r]

15. Shen, J R., Ikeuchi, M. & Inoue, Y. (1997) Analysis of the psbUgene encoding the 12-kDa extrinsic protein of photosystem II andstudies on its role by deletion mutagenesis in Synechocystis sp.PCC 6803. J. Biol. Chem. 272, 17821–17826.16. Enami,I.,Yoshihara,S.,T[r]

51. Herbomel, P., Bourachot, B. & Yaniv, M. (1984) Two distinctenhancers with different cell specificities coexit in the regulatoryregion of polyoma. Cell 39, 653–662.52. Horvath, C.M. (2000) STAT proteins and transcriptionalresponses to extracellular signals. Trends Biochem. Sci[r]

757–766.19. Bradford, M.M. (1976) A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding. Anal. Biochem. 72, 248–254.20. Laemmli, U.K. (1970) Cleavage of structural proteins du[r]

List of abbreviationsHuman papillomavirus (HPV), E6-associated protein(E6AP), 4',6-Diamidino-2-phenylindole (DAPI), greenfluorescent protein (GFP)Competing interestsThe author(s) declare that they have no competinginterests.Authors' contributionsK. V. designed and created[r]

to keep gelsolin in the calcium-free compact state, and thatthe crutial calcium binding site(s) reside in the C-terminalhalf of gelsolin perhaps involving the C-terminal helix itselfhas to be bound to release this latch. Here we provideevidence for a calcium dependent con[r]

highly responsive to induction and repression by environ-mental and xenobiotic agents in general.Recently, important advances have been made in under-standing the mechanism of action of prototype inducers thatcontrol the expression of hepatic P450 enzymes, namelyth[r]

List of abbreviationsHuman papillomavirus (HPV), E6-associated protein(E6AP), 4',6-Diamidino-2-phenylindole (DAPI), greenfluorescent protein (GFP)Competing interestsThe author(s) declare that they have no competinginterests.Authors' contributionsK. V. designed and created[r]

nuclear extracts of transfected HeLa cells, are unavail-able. In this study, we constructed and analyzed aseries of phosphomimetic mutants at sites with thehighest probability of phosphorylation (Table 1). AllV. S. Sidorenko et al. OGG1 polymorphic and phosphomimet[r]

binding to the DNA treatment with cisplatin, whereasthe presence of an overlapping GG doublet formed byone guanine inside and the other outside the p53DBSwas practically without effect.Under the conditions used in this work, the appar-ent sensitivity of p53 (or p73) DNA <[r]

with Western blots. All cysteine mutants showed detect-Fig. 2. Western blot analysis of overexpressed GlcNAc kinase andcysteine mutants in E. coli BL21. Supernatants after cell lysis wereseparated by SDS-polyacrylamide gel electrophoresis and blotted ontonitrocellulose as descri[r]

tuent of BRACO-19 from an aniline to a difluoroben-zylamine group was also carried out to circumvent theproblem of cellular uptake of BRACO-19 [35]. Thereare reports of interaction studies between G-quadru-plex DNA and the perylene diimide PIPER (Fig. 2)[36]. This molecule[r]

crosslinking and ChIP using anti-rabbit serum (negative control) or an antibody to NF-jB. Relative values of immunoprecipitated DNA wereassessed by real-time PCR (n = 3). (a) Amplification of a coding region part of the intron-less gene encoding GAPDH, which should show nopromote[r]

olytic processing, so that p105 and p100 are sometimes called precursors of p50 andp52. I κBγ can also be generated by proteolytic processing from p105. Full-lengthp105 and p100 act as IκB as well (Naumann et al., 1993).3 General Biological Role of NF-κBNF-κB transcription factors prom[r]

MINIREVIEWEvolutionary changes to transthyretin: structure–functionrelationshipsP. Prapunpoj and L. LeelawatwattanaDepartment of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, ThailandIntroductionTransthyretin is a major protein in extrace[r]

transcription factor. One example of this is seen in theobservation that the apolipoprotein AI gene expressionin liver depends on the interactions between HNF-4and HNF-3 within a hepatocyte-specific enhancer inthe 5¢ flanking region of the gene. It has been proposedthat an[r]

amino acid (Ser101) next to Trp82 in Ci1-FEHIIa isbelieved to be important for determining the orienta-tion of the Trp82 residue. The Glc moiety of Suc inCi1-FEHIIa occupied a position clearly different [25]Fig. 5. Models of the active sites of the six putat[r]