ELECTROCHEMICAL DETECTION OF NUCLEIC ACIDS

Kizek R: The determination of avidin in genetically modified maize byvoltammetric techniques. Plant Soil Environ 2007, 53:345-349.12. Banks CE, Compton RG: Edge plane pyrolytic graphite electrodes inelectroanalysis: an overview. Anal Sci 2005, 21:1263-1268.13. Erdem A: Nanomaterial-based e[r]

(dsDNA), or attachment of covalent tags to either the targetDNA or signalling probes. Covalent modification (labelling)of DNA with redox-active moieties is a particularly promisingapproach owing to unambiguous discrimination between theprobe and target strands. Labelled DNA can be prep[r]

Mitchell, P.S., Parkin, R.K., Kroh, E.M., Fritz, B.R., Wyman, S.K., Pogosova-Agadjanyan,E.L., Peterson, A., Noteboom, J., O'Briant, K.C., Allen, A., Lin, D.W., Urban, N.,Drescher, C.W., Knudsen, B.S., Stirewalt, D.L., Gentleman, R., Vessella, R.L.,Nelson, P.S., Martin, D.B., Tewari, M., 2008. Procee[r]

plant recipients. Clin Infect Dis 2001, 33:1358-1362.30. Griffiths PD, Ait-Khaled M, Bearcroft CP, Clark DA, Quaglia A, Dav-ies SE, Burroughs AK, Rolles K, Kidd IM, Knight SN, Noibi SM, CopeAV, Phillips AN, Emery VC: Human herpesviruses 6 and 7 aspotential pathogens after liver transplant: prospecti[r]

plant recipients. Clin Infect Dis 2001, 33:1358-1362.30. Griffiths PD, Ait-Khaled M, Bearcroft CP, Clark DA, Quaglia A, Dav-ies SE, Burroughs AK, Rolles K, Kidd IM, Knight SN, Noibi SM, CopeAV, Phillips AN, Emery VC: Human herpesviruses 6 and 7 aspotential pathogens after liver transplant: prospecti[r]

using microarrays [29] and quantitative real-time PCR[30], were used to help determine functionality (seereview by Clokie et al [31]).Cyanophage strain S-PM2 was originally isolated byplaque assay from coastal water off Plymouth, UK andbelongs to the family Myoviridae, a group of double-stran[r]

2+and was readily detectable in the presence of Mg2+andNi2+cations, the strand transfer activity was strictly dependent only onMn2+. M-PMV integrase displays more relaxed substrate specificity thanHIV-1 integrase, catalyzing the cleavage and the strand transfer ofM-PMV and HIV-1 long-terminal[r]

3- 3- One of 4 types of basesOne of 4 types of bases (all (all containing nitrogen): containing nitrogen): - Adenine- Adenine- - Thymine (Only in DNA)Thymine (Only in DNA)- Cytosine- Cytosine- Guanine- GuanineRNARNA Nucleotides NucleotidesComposition ( 3 parts):Compositi[r]

the manuscript. All authors read and approved the finalmanuscript.AcknowledgementsThis study was partly funded by a Natural Environmental Research Council (NERC) CONNECT B grant, GR3/CO058, awarded jointly to W.H.W. and Cytocell Ltd.: CONNECT B is a scheme designed to encourage collabora-tion betwee[r]

Zahra Lamzira, Abdeslam Asehraou, Dulce Brito, Manuella Oliveira, Mohamed Faidand Cidalia PeresReducing the Bloater Spoilage during Lactic Fermentation of Moroccan Green Olives (4) 373Davorka Breljak, Andreja Ambriovi}-Ristov, Sanja Kapitanovi}, Tamara ^a~evand Jelka GabrilovacComparison o[r]

plete ethanol removal after wash steps will cause the problemsdescribed earlier for ethanol precipitation (discussed below underthe question What Are The Fundamental Steps Of DNA Purifica-tion?). Ethanol must be completely removed from the samplesafter wash steps to avoid problems such as diff[r]

Exosomes, small-membrane vesicles, are secreted by cells and include several types of proteins and nucleic acids. Exosomes transfer cellular information derived from donor cells and are involved in various physiological and pathological events, such as organ-specific metastasis.

Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, can be secreted by most cell types and released in perhaps all biological fluids. EVs contain multiple proteins, specific lipids and several kinds of nucleic acids such as RNAs and DNAs.

23. Dovbeshko GI, Chegel VI, Gridina NY, Repnytska OP, Shirshov YM,Tryndiak VP, Todor IM, Solyanik GI: Surface enhanced IR absorption ofnucleic acids from tumor cells: FTIR reflectance study. Biopolymers 2002,67(6):470-86.24. Yamada T, Miyoshi N, Ogawa T, Akao K, Fukuda M, Ogasawara T,Kitagaw[r]

8 November 2006)doi:10.1111/j.1742-4658.2006.05603.xThis minireview looks at the latest trends in the use of nanoparticles (NPs)in electrochemical biosensing systems. It includes electrochemical characteri-zation of NPs for use as labels in affinity biosensors and other ap[r]

2/CNT/Pt electrode with glucose oxidase (GOx) presents a high sensitivity of 0.24 ␮AmM−1cm−2toglucose in the range of 0.006 mM to 1.5 mM with a response time of less than 3 s and a detection limit of5.7 ␮M at 3 signal/noise ratio.© 2008 Elsevier B.V. All rights reserved.1[r]

Nucleic AcidsNucleic AcidsDNA & RNADNA & RNAWhat are they ?What are they ?The 4The 4thth type of type of macromoleculesmacromoleculesThe chemical link between The chemical link between generationsgenerationsThe source of The source of genetic genet[r]

cytochromes haem-containing proteins. Some react with oxygendirectly; others are intermediates in the oxidation of reducedcoenzymes. Unlike haemoglobin, the iron in the haem ofcytochromes undergoes oxidation and reduction.cytochromes P450 A family of cytochromes involved in thedetoxica[r]

mercury electrodes [59]. Monitoring the changes in these signals upon duplex formation enabled the detection of hybridization [60,61]. The electrochemical signals obtained from free adenine and guanine bases decreased on binding to their complementary thymine and cytosine bases[r]

Typical Tafel polarisation curves are as shown in Figs. 1and 2.Results of the analysis of Tafel plots are presented inTables 2 and 3 below.Analysis of the inhibition efficiency (IE%) of theseadditives reveals that picric acid and H3PO4act as goodcorrosion inhibitors of<[r]