SPERM MORPHOLOGY AND DNA INTEGRITY OF FROZEN THAWED BUFFALO SEMEN TREATED WITH HEPARIN BINDING PROTEIN

cotransport of retinol through the interaction withholoRBP in plasma, in addition to that of the distribu-tion of thyroid hormones in the extracellular fluids.Experimental proceduresMaterialsHoloRBP was purified from human plasma as reportedpreviously [17]. Recombinant wild-type h[r]

mutant (DM) R297A,K299A and one in CTD (thehep2 triple mutant: K444E,R445E,R447E) (Fig. 5A).Fig. 3. The two heparin-binding domains of PKR can confer heparin-binding activity to a heterologous protein. The region between residues279 and 318 (AT[r]

standard culture media in which they have been previ-ously found to proliferate [4]. Thus, a bacterium iscurrently reported as dead when it does not yield visiblegrowth in bacteriological media for a given time [5].However, it has been suggested that the bacterialpopulations in water, when exposed t[r]

) (B), it is possible to calculate the approximate number of DNA fibers per bundle as about 500.N. Saperas et al. Protamine-like proteins and chromatinFEBS Journal 273 (2006) 4548–4561 ª 2006 The Authors Journal compilation ª 2006 FEBS 4555related sequences of their trypsin-resis[r]

The problem of calreticulin localization into the cellcontinues. Calreticulin-like immunoreactivity was detectedin the nucleus of some cells, although it seems that it is not anuclear resident protein [45,51]. An explanation for thesecontradictions may depend on the purification[r]

ATP stimulated inhibition of E2F1 by MDM2 C. Stevens et al.4882 FEBS Journal 275 (2008) 4875–4886 ª 2008 The Authors Journal compilation ª 2008 FEBSunfolded mutant p53, MDM2 and HSP90 form aninactive trimeric complex [19]. Further, MDM2–HSP90–carboxyl terminus of HSC70-interacti[r]

the effect of equimolar amounts of DS added to the cationicpeptides in RDAs. The antibacterial activity of all peptideswas completely inhibited by DS (data not shown), andsimilar results were obtained with heparin. Thus, theseexperiments demonstrated, although indi[r]

Ovarian/Testicular Shielding: Radiation shielding is routinely done in children treated with abdominal or pelvic radiation, unless the shielding will increase the chances of cancer relapse. For boys, the doctor will place lead-lined shields over one or both testicles. For girls,[r]

FIGURE 31.2 Structure and function of DnaK: (a) Domain organization and structure of the Hsp70 family mem-ber, DnaK.The ribbon diagram on the lower left is the ATP-binding domain of the DnaK analog, bovine Hsc70;bound ADP is shown as a stick diagram (purple)[r]

virion. In this structural study, the fiber-binding sitewas in a ‘bound’ conformation, although no fiber waspresent. The most likely explanation for this observa-tion is that the switch from the fiber ‘bound’ to‘unbound’ states of the penton base (or vice versa) isnot only dependent on th[r]

and low potential pathways” Free Radic Res, 36, pp.381- 387.28. Taanman J.W., Street R. H., and L. Nw (1999), “The mitochondrial genome :structure, transcription, translation and replication”, Biochim Biophys Acta,1410 (2), pp.103-123.29. Tan D.J., Bai R.K., Wong L.J. (2002), “C[r]

ands were extracted from Papilio RBP purified from the solublefraction of the dark-adapted retina. Extraction and analysis werecarried out under dim red light as follows. Purified protein was firstmixedwith2Mhydroxylamine and cold 90% methanol to convertaldehydes, if any, to[r]

whereas FEZ1 e xpression is m ore restricted, although notexclusively, to the brain.Developmental pattern of expression of murine Nbr1Using an a ntisense cDNA probe to murine Nbr1 encodingexons 16±3¢ UTR, the developmental e xpression pattern ofFig. 2. Co-immunoprecipitation experiment[r]

3-binding activity of xCTBP/xALDH1 was markedlyinhibited by NAD+, NADH, progesterone and 11-deoxy-corticosterone, as well as iodothyronines and Triac, but notby NADP+, NADPH, disulfiram and retinal. On the otherhand, the ALDH activity was inhibited by NADH,NADPH, di[r]

lates presynaptic function. Neuron 19, 391–402.6. Ford, J.C., al-Khodairy, F., Fotou, E., Sheldrick, K.S., Griffiths,D.J. & Carr, A.M. (1994) 14-3-3 protein homologs required for theDNA damage checkpoint in fission yeast. Science 265, 533–535.7. van Heusden, G.P., Griffiths, D.J., Ford, J.[r]

interaction is e nhanced several orders of magnitude and theinhibition rate approaches the v alue observed for thrombininhibition by antithrombin (AT) in the p resence o f heparin,indicating that HCII may be an interesting anticoagulantand antithrombotic agent. Increased plasma[r]

C (Bacillus stearothermophilus), respectively for 24-48 h. Colony forming units were finally counted to determine the number of survivors of bacteria. Scanning electron microscopy (SEM). Samples were imaged using field-emission scanning electron microscopy at a low kinetic energy of[r]

RESEARC H Open AccessEffects of GnRH agonist treatment onsteroidogenesis and folliculogenesis in the ovaryof cyclic micePadmasana Singh, Amitabh Krishna*AbstractBackground: GnRH analogs (both agonist and antagonist) have been extensively used for clinical applications,following[r]

repA mRNA of plasmid RI at the 5¢-UUACU-3¢sequence is very important to fine tuning the CopB ⁄RepA ratio and the replication efficiency of the plas-mid. Cleavage at these sequences in other mRNAsmay have an important role in the protein synthesisand cell growth inhibition m[r]

The rs2282679 A>C polymorphism in the vitamin D binding protein gene is associated with lower circulating levels of vitamin D. We investigated associations of this SNP with colorectal cancer (CRC) risk and survival and whether the associations vary by dietary vitamin D intake and tumor molecular phe[r]