EXPRESSION OF DEFENSE RESPONSIVE GENE

Nutrition is believed to be a primary contributor in regulating gene expression by affecting epigenetic pathways such as DNA methylation and histone modification. Resveratrol and pterostilbene are phytoalexins produced by plants as part of their defense system.

of TNF-a for3, 6, 9, 12 or 24 h. After total RNA isolation, caspase-1, IRF-1, p73 and GAPDH mRNA levels were analyzed by semiquantitative RT-PCR. C,untreated control cells. Numbers at the top of the lower panel indicate the relative amount of the p73 PCR product. (B,C) Immunoblo[r]

In this article, we aimed to characterize the diffuse gastric cancer cells with MRI and transcriptomic data. We hypothesized that gene expression pattern is associated with the phenotype of the cells and that the heterogeneous enhancement pattern and the high tumorigenicity of SNU484 can be modulate[r]

MicroRNAs are known to inhibit gene expression by binding to the 3′UTR of the target transcript. Downregulation of miR-223 has been recently reported to have prognostic significance in CLL. However, there is no evidence of the pathogenetic mechanism of this miRNA in CLL patients.

more detailed interrogation is required to confirm Hunk as a Wnt target gene.
Studies using Xenopus embryos have shown that Hunk has the ability to modulate Wnt signalling. We used qRT-PCR analysis to examine this in the intestine and demonstrated a significant reduction in expression<[r]

The heterogeneous behavior of patients with melanoma makes prognostication challenging. To address this, a gene expression profile (GEP) test to predict metastatic risk was previously developed. This study evaluates the GEP’s prognostic accuracy in an independent cohort of cutaneous melanoma patient[r]

Molecular characterization of circulating tumor cells (CTC) is promising for personalized medicine. We aimed to identify a CTC gene expression profile predicting outcome to first-line aromatase inhibitors in metastatic breast cancer (MBC) patients.

feature can be very important for monitoring of miRs se- creted from the expanding EBC tissue.
Work by others involving 76 BC samples and 10 con- trol tissues and utilizing microarray technique followed by Northern blot validations demonstrated that miRs are deregulated in BC with a defin[r]

MicroRNAs are single-stranded non-coding RNA sequences of 18 - 24 nucleotides in length. They play an important role in post-transcriptional regulation of gene expression. Evidences of microRNA acting as promoter/suppressor of several diseases including cancer are being unveiled.

This study assessed the expression of an important TLR9 gene in spontaneous bovine sub-clinical and clinical mastitis caused by E. coli. The total RNA was isolated from milk somatic cells converted as cDNA using oligo (dT) primers. Relative quantitation of mRNA of TLR9 was analysed by RT-qPCR system[r]

Hepatocellular carcinoma (HCC) is a lethal disease, while the precise underlying molecular mechanisms of HCC pathogenesis remain to be defined. MicroRNA (miRNA), a class of non-coding small RNAs, can post-transcriptionally regulate gene expression.

Recently, copy number alteration (CNA) of 9p24.1 were demonstrated in 10% of diffuse large b-cell lymphoma (DLBCL), with gene expression and mutation profiles that were similar to those of primary mediastinal large B-cell lymphoma (PMBCL).

High HOX gene expression has been described in many cancers, including oral squamous cell carcinoma and the functional roles of these genes are gradually being understood. The pattern of overexpression suggests that inhibition may be useful therapeutically.

Increased mesenchymal–epithelial transition factor gene (c-MET) expression in several human malignancies is related to increased tumour progression. The aim of the present study is to explore the relationship between immunohistochemical expression of c-MET in colorectal carcinoma (CRC) and the clini[r]

Hepatocyte nuclear factor-3β (HNF-3β) plays a critical role in hepatocyte differentiation and controls liver-specific gene expression during the development of hepatocellular carcinoma (HCC), but the molecular basis of this process has not been fully elucidated.

The purpose of this study was to evaluate invasive and metastatic potential of urothelial cancer by investigating differential expression of various clock genes/proteins participating in the 24 h circadian rhythms and to compare these gene expressions with transcription of other cancer-associated ge[r]

Strongly increased uptake of glucose is a hallmark of solid malignant tumors. This phenotype can be triggered by hypoxia-induced gene expression changes or can occur independently of hypoxia as a consequence of malignant transformation itself, and is often referred to as the Warburg effect.

Dysregulation of the long non-coding RNA small nucleolar RNA host gene 16 (lncRNA SNHG6) has been found in multiple cancers. However, a definite conclusion on the clinical value of lncRNA SNHG6 expression in human cancers has not been determined.

the zebrafish system. Experimental manipulation of embryos can be done by techniquessuch as microinjection of biologically active molecules, microbead implantation, celltransplantation, fate mapping and cell-lineage tracing (Stainier et el., 1993; Mizuno et al.,1999; Holder and Xu, 199[r]

MicroRNAs (miRNAs or miRs) are small molecules known to be involved in post-transcriptional gene expression. Many of them have been shown to influence risk for various diseases.