STABLE ISOTOPE LABELLING WITH AMINO ACIDS IN CELL CULTURE FOR HUMAN EMBRYONIC STEM CELL PROTEOMIC AN...

7. Summary and future directions of the glioma stem cell field
In our view, the cancer stem cell model has had a positive effect on cancer research, leading to a close examination of tumor cell heterogeneity brought about by differentiation, as well as o[r]

Results and Discussion
Cell Migration on Exposure to a Magnet Field
In our previous work, we demonstrated that SH-SY5Y cells, cultured with a cell culture medium modified with PF-127 coated CNTs, are able to migrate under the effect of an external magnet[r]

Osteosarcoma is the most common type of solid bone cancer and the second leading cause of cancer-related death in pediatric patients. Many patients are not cured by the current osteosarcoma therapy consisting of combi- nation chemotherapy along with surgery and thus new treatments are[r]

with Fc RI or aggregation of Fc RI, for the time periods indicated. The conjugated antibody, TNP7- MAR F(ab´) 2 , functions as a multivalent antigen recognized by the Fc RI-bound IgE but also rec- ognizing bound 2.4G2 rat Ab. 13 Aggregation with 2.4G2 rat Ab together with<[r]

Recent advances in bioimaging and automated analysis methods have enabled the large-scale systematic analysis of cellular dynamics during the embryonic development of Caenorhabditis elegans. Most of these analyses have focused on cell lineage tracing rather than cell shape dynamics.

Mass spectrometry-based proteomics is an alternative method for the analysis of protein profiles and provides an extensive approach to analyze expressed proteins and dis- cover potential biomarkers for diseases. The development of MS based technology has provided the opportunity[r]

A large group at the Vienna University of Agriculture has specialized in a number of aspects of mammalian cell culture. Novel methods of cell hybridiza- tion have been developed [89].As mentioned earlier, a highly instrumented pilot plant has been established, m[r]

The role of sphere-forming culture in enriching subpopulations with stem-cell properties in hepatocellular carcinoma (HCC) is unclear. The present study investigates its value in enriching cancer stem cells (CSCs) subpopulations and the mechanism by which HCC CSCs are maintained.

Human immunological memory is a hallmark of the adaptive immune system and plays an important role in the development of effective immune responses against tumors. In the present study, we aimed to determine the frequencies of CD8+ memory T cell subsets including T stem cell memory (TSCM) in tumor-d[r]

Human Mesenchymal Stem Cells (hMSCs) undergo senescence in lifespan. In most clinical trials, hMSCs experience long-term expansion ex vivo to increase cell number prior to transplantation, which unfortunately leads to cell senescence, hampering post-transplant outcomes.

K.T. Meyer is regarded as an efficient alternative to cell culture for biomass production due to its fast growth and stable metabolite production. To determine optim[r]

Nitrogen has been associated to lettuce fresh weight accumulation. In this way, different bio stimulants formulations containing amino acids have been commercially offered although the yield increase has not been properly supported. The aim of this work was a) to quantify the changes in lettuce fres[r]

Exosomes or extracellular vesicles have the potential as a diagnostic marker for various diseases including cancer. In order to identify novel exosomal markers for prostate cancer (PC), we performed proteomic analysis of exosomes isolated from PC cell lines and examined the usefulness of the marker[r]

detected this phenomenon in MDA-MB-231, an ER- negative BC cell line (Additional file 1: Figure S3).
Activation of YB-1 suppresses Sox2 expression
To address if YB-1 activation, as evidenced by phosphor- ylation at its serine-102 residue [31,32], modulates Sox2 expression, we treat[r]

Developmental Potency
The term potency is used to indicate a cell's ability to differentiate into specialized cell types. The current lack of knowledge about the molecular nature of potency requires the experimental manipulation of stem cells[r]

In this regard, the ability to generate cells of the hemat- opoietic system has considerable potential in several areas of clinical and experimental medicine as they can recon- stitute the entire blood system and can serve as primary targets in gene therapy in treating in[r]

The law defines an embryo as “a cell (except for a germ cell) or cells which has/have potential to grow into an individual through the process of development in utero of a human or an animal and has/have not yet begun formation of a placenta.”
Supplementary[r]

Abstract
As mass-spectrometry-based quantitative proteomics approaches become increasingly powerful,
researchers are taking advantage of well established methodologies and improving instrumentation to pioneer new protein expression profiling methods. For example, pooling several proteo[r]

Mantle cell lymphoma (MCL) is a rare and aggressive form of non-Hodgkin’s lymphoma. Bortezomib is the first product to be approved for the treatment of patients with previously untreated MCL, for whom haematopoietic stem cell transplantation is unsuitable, and is used in combination with rituximab,[r]

Endoderm Differentiation ...161 BIBLIOGRAPHY……….………...…..164 TRANG 6 SUMMARY Human embryonic stem cells hESCs are derived from the inner cell mass of a 5-day old blastocyst.. hESCs posse[r]