)1) or chloroquine (0.2 mM)at37°C for 3 h before harvest. Protein lysate (50 lg) was separated by SDS ⁄ PAGE and transferred toa nitrocellulose membrane. The western blot analyses were performed as described in (A). (D) Quantification of the expression levels of thewt or mutant ZIP1-[r]
the E. coli enzyme, was found to be located near the activesite. The third conserved c ysteine, C72 in the M. tuberculosisenzyme, corresponding to C176 in the E. coli enzyme, islocated near the S-AdoMet binding site but far from theactive site. T herefore, it was reasonable to postulate animportant[r]
interaction energy function precalculated on a grid. Tovalidate the docking protocol, it was first applied to threedifferent experimental protein–heparin complexes depositedat the Protein Data Bank (PDB) files [31], PDB code 1bfc,1azx, 1e0o [32–34]. These proteins were energy-minimizedto allow relaxat[r]
cules. The data suggest that ArgEE could be used as anadditive to prevent inactivation and aggregation of heat-labile proteins.Keywords: arginine; arginine ethylester; lysozyme; proteinaggregation; thermal inactivation.Protein a ggregation is a serious problem for both biotech-n[r]
SEISMIC RESPONSE OF REINFORCED CONCRETE BEARING WALLS, 2DAND 3D F.E. SIMPLIFIED ANALYSISFrédéric Ragueneau1,Géraldine Casaux2,and Jacky Mazars3, Member ASCEABSTRACTThis paper deals with the behavior of reinforced concrete bearing walls subjected to seismic loading.The presentation focu[r]
on of target (substitution of terminal amino acid of peptidoglycan subunit) Bacitracin Cell wall Prevents addition of cell-wall subunits by inhibiting recycling of membrane lipid carrier Not defined C Macrolides (erythromycin) Protein synthesis Bind to 50S ribosom[r]
vs. time (which is measured using the optical deflec-tion technique [3] and is used to obtain the velocity ofthe vapor front) shown in Fig. 5. The onset of ablationobtained using these two methods is shown in Fig. 6aand b. Both figures show that, when the laser fluence ishigher than 5.2 J/cm2, t[r]
during tumor development are point mutations and large deletions. Point mutations in the coding region of tumor-suppressor genes will frequently lead to truncated protein products or otherwise nonfunctional proteins. Similarly, deletions lead to the loss of a functional product and som[r]
vated FNR is a mixture of apoFNR with one and twodisulfides, and the disulfides are the same as in aerobicapoFNR (Cys16 ⁄ Cys20 and Cys23 ⁄ Cys29).DiscussionSignificance of apoFNR[4Fe-4S]ÆFNR is converted rapidly to apoFNR afterexposure to air and a lag phase of about 2 min. Thelag[r]
way. For T = 13 K, thermal emission is negligible. Forthat temperature, the DLTS amplitude was calculated as afunction of reverse voltage, by fitting the average electronbinding energies of the s- and p-levels and by using tun-neling emission data from Ref. [2]. For the highesttemperatu[r]
decrease in phosphorylase a reverses the inhibition ofglycogen synthase phosphatase [1].Correspondence to L. Agius, School of Clinical Medical Sciences,The Medical School, University of Newcastle upon Tyne,Newcastle upon Tyne, NE2 4HH, UK.Fax: + 44 191 2220723, Tel.: + 44 191 2227033,E[r]
sired s ource components when using a single-channel post-filtering approach, the enhancement of the beamformer out-put by multichannel postfiltering produces a significantly re-duced level of residual transient noise without further dis-torting the desired signal components. We no[r]
[29] and surfactants [30], were found to promote thecorrect folding of proteins. When using these additives itis important to use the appropriate nondenaturing con-centration, but this may be difficult because the nativestate is easily destabilized in the presence of theseadditives.18On[r]
single-stranded. Such conditions are found as standardin telomeres, where there is a single-stranded overhang,and during replication, when the strands must be sepa-rated; G-quadruplex formation has been proposed forboth of these. One other, rarer, occasion when DNA issingle-stranded is a G-lo[r]
For information on the specific model types and associated parameters, refer to morecomplete documentation.E.4 Analysis TypesE.4.1 Operating Point AnalysisThe DC analysis portion of Spice determines the operating point of the circuit withinductors shorted and capacitors opened. An oper[r]
Using the Explorer IDEAfter a plot has been selected, you can change its properties via the Plot Propertiesdialog accessible from the right-mouse button within the plot window. The Plot Prop-erties dialog allows you to set generic, axis, and signal attributes.Generic plot attributes include the plot[r]
suggests that at least two processes were involved in thethermal inactivation of the mutants. Similar phenomenahave been previously observed for other thermostablemutants (N212D and E160G/N212D) [12] and may beexplained by the multiple-exponential state in the unfoldingkinetics [16]. T[r]
insensitive to the addition of exogenous PG-C16:1(3t) [8],raising the possibility that PG-C16:1(3t) deficiency was notresponsible for PSII inactivation. The extensive geneticanalysis performed in the present study nevertheless definesa single nuclear mutational event that governs bothphe[r]
cytokines and COX enzymes by quantitative RT-PCR. Twenty-three tMCAo animals were studied with diffusion and T2weighted MRI within the first 24 hours, and ten of the SHRs underwent follow-up MRI six days later. Thirty-three SHRswere given 5-bromo-2'-deoxy-uridine (BrdU) twice daily on Day 4 t[r]
tion in HNK-siTRPC4.ABCDEFig. 2. Calcium uptake and mediators in HNK and RCC cells. (A) Ca2+uptake in response to VEGF by HNK and RCC cells. HNK and RCCcells were preloaded with fluo-4 acetoxymethyl ester and treated with 10 ngÆmL)1VEGF. Ca2+uptake was measured at 10 s intervals byvideofluorescence im[r]