From all the data supported in this thesis, we report the first highly selective bioimaging fluorescent probe for mESCs (CDy9) via high throughput screening using DOFLA. Although several mESC fluorescent probes (CDy1, CDg4, CDb8) have been published previously, none of th[r]
Developmental Potency The term potency is used to indicate a cell's ability to differentiate into specialized cell types. The current lack of knowledge about the molecular nature of potency requires the experimental manipulation of
Methods:The specificity, sensitivity and biostability of cy-apt 20 in detecting gastric cancer were assessed by binding assay, cell fluorescence imaging, and in vivo tumor imaging in ani[r]
phenotypes of precancerous stem cells, meanwhile they have their unique profiles. Castro and colleagues found that 126 genes were upregulated and 21 genes were down- regulated in DCIS compared to IDC. Therefore, precan- cerous stem cells of DCIS exhibit different[r]
It is important that hES-DCs are also functionally normal for future applications. Therefore we analyzed the func- tional markers HLA-DR (MHC-II), B7.1 (CD80), and B7.2 (CD86) typically expressed by mature DCs. The antigen presenting cell surface marker, HLA-DR present on mature DCs is critic[r]
Stem cell research has been extensively explored worldwide to enhance human health. Nevertheless, there is currently no full understanding of the perception toward stem cell research among veterinarians in India. This study aimed to assess the perception toward stem cell research, and associated fac[r]
68 3T TRANG 8 DANH MỤC CÁC TỪ VIẾT TẮT ACEI Angiotensin converting enzyme inhibitor Ức chế enzym chuyển đổi agiotensin CBE Cord blood- derived embryonic- like stem cell Tế bào gốc giống [r]
To describe the quality of cord blood stem cell stored in Stem Cell Bank of National Institute of Hematology and Blood Transfusion, to assess the initial results of cord blood transplantation for acute leukemia treatment.
Stem cell protein Piwil1 functions as an oncogene in various tumor types. However, the exact function and mechanism of Piwil1 in endometrial cancer remains unclear. Our results suggested that stem cell protein Piwil1 play important roles in regulating EMT and the acquisition of stem-like properties[r]
hyperglycemia was attenuated. It was observed that some of the transplanted mice developed teratomas, suggesting the cell preparation was heterogenous and contaminated with ESCs that could differentiate into all cell types. In contrast, differentiation of mouse[r]
The floor of this cavity is formed by the embryonic disk, which is composed of a layer of prismatic cells, the embryonic ectoderm, derived from the inner cell mass and lying in appositio[r]
Both of these questions are surprisingly thorny. Consider the matter of potency: the usual definition is the ability to generate cells of other types, but there are two problems with this. First, there are clearly cells in the body that gener[r]
Cells were washed and analyzed on a FACS Calibur instru- ment (BD Biosciences). Experimental autoimmune encephalomyelitis EAE was induced in C57BL/6 female mice at 10-12 weeks of age. Mice were injected with 200 ng pertussis toxin (Sigma-Aldrich, St. Louis, MO) i.v. on days 0 and 2[r]
Estrogen receptor a and b (ER a and b ) have been identified in colon tissue [15,39,40]. In the normal colon mucosa, there are no significant differences in the pro- tein expression of ER a and b between men and women [40]. Epidemiological data on taking home replacement[r]
166 background signals. Only in recent years have cameras become sensitive enough to detect the faint light emis- sions of the luciferase reaction from within intact animals. The most useful detectors are back-illuminated, cooled, charge-coupled device (CCD) cameras that have very[r]
adult human testis, providing another strategy for obtaining genetically identical stem cells. Umbilical cord blood stem/progenitor cells are associated with less graft- versus-host disease compared to marrow stem cells. They have less HLA restr[r]
of skin, intestine, and blood cells throughout the lifespan. These resident stem cells are now identified in a variety of organs, i.e., epithelia of the skin and digestive system, bone marrow, blood vessels, brain, skeletal muscle, live[r]
In vivo optical imaging After injecting DiD-labeled monocytes into FVB/n con- trols, progressively increasing fluorescence was noted in the liver, spleen, and lungs over 24 hours. (Figure 3). The same pattern was observed in MMTV-PymT mice. In addi- tion,[r]
down-regulation of COX-2 might suppress cell tube for- mation and migration in HUVEC. Effect of COX-2 on angiogenesis related molecules Using cDNA microarray, genes were identified differen- tially expressed between different transfected SGC7901 cells. Compared[r]
BAP1 gene expression was silenced in another LAC cell line NCI-H1650, in order to test the inhibitory effect of BAP1 on cell migration and invasion, as well as cell cycle regulation.. Re[r]