IDENTIFICATION OF GENE AND PROTEIN TARGETS TO DRUGS AND VACCINE DEVELOPMENT

Development of resistance to conventional drugs and novel biological agents often impair long-term chemotherapy. HMGA gene overexpression is often associated with antineoplastic drug resistance and reduced survival.

Immunohistochemistry
The TMA recipient block was sectioned on a routine microtome machine. The IHC assay using EGFR rabbit monoclonal antibody (EGFR.25, Leica Biosystems Newcastle Ltd, Newcastle, UK) was performed with the Ventana iView DAB Detection Kit on a BenchMark XT automated staining system[r]

This non-systematic review article aims to summarise the progress made in understanding the functional consequences of microRNA (miRNA) dysregulation in prostate cancer development, and the identification of potential miRNA targets as serum biomarkers for diagnosis or disease stratification.

Genomic DNA extraction, PCR (Polymerase Chain Reaction) amplification of the TP53 exons 2 – 11, and mutational analysis by direct sequencing
DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples on histological sections was per- formed. For microdissection, appro[r]

Inflammasomes are reported to be abnormally expressed and activated in several malignancies and play important roles in tumor development. The present study was designed to investigate the expression and function of the NLR family pyrin domain containing protein 3 (NLRP3) inflammasome in oral squamo[r]

Hepatitis B Virus (HBV) X protein (HBx) is known to be involved in the initiation and progression of hepatocellular carcinoma (HCC) through modulation of host gene response. Alterations in miRNA expressions are frequently noted in HCC.

Estrogen (17β-estradiol) promotes the survival and proliferation of breast cancer cells and its receptors represent important therapeutic targets. The cellular actions of estrogen are mediated by the nuclear estrogen receptors ERα and ERβ as well as the 7-transmembrane spanning G protein-coupled est[r]

EGFRvIII is a mutant form of the epidermal growth factor receptor gene (EGFR) that lacks exons 2–7. The resulting protein does not bind to ligands and is constitutively activated. The expression of EGFRvIII is likely confined to various types of cancer, particularly glioblastomas.

The cancer stem cells (CSCs), a small subpopulation of cells in tumor are responsible for the tumor initiation, growth, recurrence and metastasis of cancer, as well as resistance of cancers to drugs or radiotherapy. CSCs are an important target for the development of novel strategies in cancer treat[r]

The aim of this study was to isolate, characterize and identify endophytic bacteria from plants growing along the stream banks in Hot Springs, South Dakota. The bacterial endophytes were isolated, identified and screened in vitrofor morphological features (Gram stain, Gram morphology, and colony mor[r]

P53 gene mutation is widely known as one of the determinants of impaired chemosensitivity. p53 is a tumor-suppressor protein in humans encoded by the TP53 gene. Some studies have shown that TP53 gene mutation and accumulation of the p53 protein are closely related with serum anti-p53 antibody positi[r]

DatabaseThe nucleotide sequence of the ldhB genefrom L. lactis MG1363 has been submittedto the GenBank database under theaccession number AY236961(Received 2 August 2007, revised 20 Sep-tember 2007, accepted 21 September 2007)doi:10.1111/j.1742-4658.2007.06115.xLactococcus lactis FI9078, a co[r]

To evaluate the anticancer effect of measles vaccine virus in combination with Nimotuzumab in vitro. To evaluate the anticancer effect of measles vaccine virus in combination with Nimotuzumab on nude mouse model with head and neck cancer (in vivo).

Methods: The level of MSK1 phosphorylation at Thr581 was detected by the immunohistochemical analysis in NPC tissues and normal nasopharynx tissues, and its correlation with LMP1 was analyzed in NPC tissues and cell lines. Using MSK1 inhibitor H89 or small interfering RNA[r]

The present study was designed to express full length G7L protein of GTPV using baculovirus expression vector system (BEVS) in insect cells and evaluate its diagnostic potential for detection of antibodies to GTPV. Initially, G7L gene of GTPV-Uttarkashi strain was amplified and cloned into BEVS dono[r]

To describe EEG and relationship between EEG and clinical features in patients with schizophrenia. To assess the frequency of allele and genotypic distribution of ZNF804A gene polymorphism rs1344706 and COMT gene polymorphism rs165599 in patients with schizophrenia.

Targeting cell metabolism offers promising opportunities for the development of drugs to treat cancer. We previously found that the fatty acyl-CoA synthetase VL3 (ACSVL3) is elevated in malignant brain tumor tissues and involved in tumorigenesis.

Previous studies had highlighted hScrib as a potential biomarker for HPV-16 induced malignancy [19,28,34]. We reasoned that if PP1 γ was also regulated directly by hScrib, this should be similarly affected in HPV-16 in- duced malignancy. In order to investigate this we per- formed IHC analysi[r]

The phenomenon of chemotherapy-resistant cancers has necessitated the development of new therapeutics as well as the identification of specific prognostic markers to predict the response to novel drugs.

Genetic testing for BRCA1 and BRCA2 has led to the accurate identification of individuals at higher risk of cancer and the development of new therapies. Approximately 10-20% of the genetic testing for BRCA1 and BRCA2 leads to the identification of variants of uncertain significance (VUS), with highe[r]