PREPARATION AND STANDARDIZATION OF BASE AND ACID SOLUTIONS

Preparation of K-L Strips: Cut Whatman #3 filter paper into1.5cm × 7cm strips. Autoclave for 15 min at 15 psi pressure–121°C.Aseptically dip each strip into a sterile solution containing 1000U ofpurified diphtheria toxin/mL. Drain off excess liquid. Preparation of Medium:[r]

Preparation of Carbohydrate Solution: Add carbohydrate todistilled/deionized water and bring volume to 100.0mL. Adonitol, ara-binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac-tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,trehal[r]

Phenol Red 0.02gSubstrate solution 50.0mLpH 6.5 ± 0.2 at 25°CSubstrate Solution:Composition per 50.0mL:Substrate 0.1gPreparation of Substrate Solution: Add substrate to distilled/de-ionized water and bring volume to 50.0mL. Use gelatin, aliphatic acids,or any alkalogenic carbon source.[r]

CHO Medium Base(Carbohydrate Medium Base)Composition per liter:Pancreatic digest of casein 15.0gYeast extract 7.0gNaCl 2.5gAgar 0.75gSodium thioglycolate 0.5gL-Cystine 0.25gAscorbic acid 0.1gBromthymol Blue 0.01gpH 7.0 ± 0.2 at 25°CPreparation of Medium: Add compon[r]

Composition per liter:Cereal, precooked mixed 100.0gAgar 15.0g© 2010 by Taylor and Francis Group, LLCCetrimide HiVeg Agar Base with Glycerol and Nalidixic Selective Supplement 343Preparation of Medium: Add components to distilled/deionizedwater and bring vol[r]

VIETNAM UNIVERSITY OF COMMERCEFACULTY OF ENGLISHGROUP DISCUSSIONENGLISH 1Topic: Biggest problems in your study and suggested solutionsGroup: 2Class:Group preparation assessment:Presentation assessment: List of students ( in the order of presenting )No1Name([r]

Fat substrate 50.0gPreparation of Fat Substrate: Corn oil, soybean oil, any cookingoil, lard, tallow, or triglycerides that do not contain antioxidants or oth-er inhibitory substances may be used. Remove free fatty acids in the fat© 2010 by Taylor and Francis Group, LLC1574 Singulospha[r]

4, anhydrous 0.024gpH 7.2 ± 0.2 at 25°CSource: This medium is available as a premixed powder from BD Di-agnostic Systems.Preparation of Medium: Add components to distilled/deionizedwater and bring volume to 1.0L. Mix thoroughly. Gently heat with mix-ing and bring to boili[r]

Actinosynnema mirum, Amycolata hydrocarbonoxydans, Amycolatop-sis mediterranei, Amycolatopsis orientalis, Catellatospora ferruginea,Dactylosporangium aurantiacum, Microbispora chromogenes, Micro-bispora thermodiastatica, Micromonospora chalcea, Microtetrasporaangiospora, Microtetraspora incanescens,[r]

of OCPs has been discontinued largely as a result of the long history ofbioaccumulation, toxicity, and high environmental pesistence. Several OCPswere selected as model analytes to evaluate the fully automated PIN-LPMEprocedure.Stock solutions of each OCP were prep[r]

KH2PO4 0.3gCaCl2 0.2gKCl 0.2gPreparation of Locke's Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclavefor 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.Preparation of Medium: Aseptically combine 700.0mL of[r]

, anhydrous 1.1mgpH 9.0 ± 0.2 at 25°CPreparation of Medium: Add tris(hydroxymethyl)aminomethanebuffer to distilled/deionized water and bring volume to 1.0L. Mix thor-oughly. Adjust pH to 9.0. Add the remaining components, except Tol-uidine Blue O. Mix thoroughly. Gently heat and[r]

C18H39N Octadecylamine 25 10.60C20H21NO4 Papaverine 25 6.40C20H24N2O2 Quinine 1 25 8.522 25 4.13C21H22N2O2 Strychnine 25 8.26C23H26N2O4 Brucine 1 25 8.28References1. Perrin, D. D., Dissociation Constants of Organic Bases in Aqueous Solution, Butterworths, London, 1965; Supplement, 1972.2. Ser[r]

Filter sterilize. Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-mation and inhalation.Preparation of Medium: Add components, except blood and se-lective supplement, to distilled/deionized water and bring volume to900.0mL. Mix thoroughly. Heat g[r]

microorganisms.Cystine Heart Agar with Rabbit BloodComposition per liter:Beef heart, solids from infusion 500.0gAgar 15.0gGlucose 10.0gProteose peptone 10.0gNaCl 5.0gL-Cystine 1.0gRabbit blood, defibrinated 50.0mLpH 6.8 ± 0.2 at 25°CSource: This medium is available as a premixed powder from BD Di-ag[r]

per 10.0mL:Yeast extract 1.0gPreparation of Yeast Extract Solution: Add yeast extract to dis-tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-ature. Sparge with 100% N2. Peptone Solution:Composition per[r]

Peptic digest of animal tissue 10.0gNaCl 5.0gBeef extract 3.0gCarbohydrate solution 100.0mLAndrade’s indicator 10.0mLpH 7.2 ± 0.1 at 25°CSource: This medium is available as a premixed powder from BD Di-agnostic Systems.Carbohydrate Solution:Composition per 100.0mL:Carbohydrate 10.0gPreparatio[r]

O to 10.0mL of HCl solution. Mix thoroughly. Add dis-tilled/deionized water and bring volume to 1.0L. Add remaining com-ponents. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 minat 15 psi pressure–121°C. Adjust pH to 7.0.Preparation of Medium: Add components, exce[r]

tose, especially the coliform bacterium Escherichia coli, appear as colo-nies with a green metallic sheen or blue-black to brown color. Bacteriathat do not ferment lactose appear as colorless or transparent light purplecolonies.Levinthal’s AgarComposition per 105.0mL: Nutrient agar, sterile 100.0mLR[r]

·4H2O 30.0mgNiCl2·6H2O 20.0mgCuCl2·2H2O 10.0mgPreparation of Trace Elements Solution: Add components todistilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add components to distilled/deionizedwater and bring volume to 1.0L. Mi[r]