VASCULAR SMOOTH MUSCLE CELLS ISOLATION CULTURE AND CHARACTERIZATION

giant cell arteritis. Protein extract is from human umbilical veinendothelial cells (HUVECs). (A) IgG reactivities to lamin of threedifferent pools of sera from three giant cell arteritis patients each (P1 toP3) and one pool from twelve healthy controls (HCs). (B) Lamin spots areexpres[r]

Chapter 049. Sexual Dysfunction (Part 2) Pathways that control erection and detumescence. A. Erection is mediated by cholinergic parasympathetic pathways, and nonadrenergic, noncholinergic (NANC) pathways, which release nitric oxide (NO). Endothelial cells also release NO, whic[r]

The adaptations to hypoxia are mediated, in part, by the upregulation of genes encoding a variety of proteins, including glycolytic enzymes such as phosphoglycerate kinase and phosphofructokinase, as well as the glucose transporters Glut-1 and Glut-2; and by growth factors, such[r]

Fig. 2. Expression pattern of tsA58T Ag in T26 ⁄ Tie2–Cre double-transgenic mice. (A) tsA58T Ag (red) was expressed in CD31-positive ECs(green) of an E9.5 T26 ⁄ Tie2–Cre double-transgenic embryo and its yolk sac. (B) tsA58T Ag (red) was expressed in CD31-positive ECs (green,left panels) an[r]

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remodeling proc ess of the airways, and not to its trigger.To our knowledge, not much attention has previouslybeen paid on Tn-C expression outside the basementmembrane area. We observed, however, that over 50%of our COPD-patients showed an increased expressionof Tn-C beyond the basement membr[r]

designed and edited the manuscript. All authors read andapproved the manuscript.Additional materialAcknowledgementsThe human ES cell (HES-3) was provided by ES cell International Pre Ltd, Singapore. This work was supported by grants from Japanese Ministry of Education, Culture, Sports,[r]

L-ornithine (a commonsubstrate for neural stem cell studies). Clearly, CNTs/CNFsplayed an important role in effectively delivering stemcells into injured sites and promoted stem cells to differ-entiate into favorable neurons to repair damaged neuraltissues.The promise of nanomaterials[r]

chymal stem cells in bone tissue engineering applications: current status and future prospects. Tissue Eng 2005, 11, 787-802.26. Ochs HD, Thrasher AJ. The Wiskott-Aldrich syndrome. J Allergy Clin Immunol 2006, 117, 725-738.27. Ohgushi H, Caplan AI. Stem cell technology and bioce[r]

showed a lower systolic BP and a steeper increase of thesystolic BP during the first month after birth than did indi-viduals that showed a higher weight at birth. The directassociation at birth and the inverse association at onemonth of life point out that the association between birth[r]

Laet A, Vaneerwinden JM, Wedel T, Baker D, Doeven-dans P, Hofker M et al. (2005) Smoothelin-a is essentialfor functional intestinal smooth muscle contractility inmice. Gastroenterology 129, 1592–1601.36 Malmqvist U, Trybus KM, Yagi S, Carmichael J &Fay FS (1997) Slow cycling of[r]

synthases of Pinus contorta and related conifers: a new class ofterpenoid cyclase. J. Biol. Chem. 269, 4012–4020.38. Hernandez,A.&Ruiz,M.T.(1998)AnEXCELtemplateforcalculation of enzyme kinetic parameters by non-linear regression.Bioinformatics 14, 227–228.39. Lange, M.B., Wildung, M.R[r]

(Fig. 6B). Thesefindings indicate that proteins homologous to ablomin arefound in several snake venoms and represent new snakevenom proteins with neurotoxin-like activities.DISCUSSIONSnake venom neurotoxins, represented by a-neurotoxinsand dendrotoxins, are thought to be found mostly inElapida[r]

fibers have a much smaller diameter than skeletal muscle cells. T-tubules are notrequired to reach the interior of the cell and therefore not necessary to transmit an actionpotential deep into the fiber. Smooth muscle fibers have a limited calcium-storing SRbut hav[r]

Adult stem cells are crucial for maintaining proper function and repair of tissues. The epithelium of the adult mammalian intestine is a constant dialog with its underlying mesenchyme to direct progenitor proliferation, lineage commitment, terminal differentiation and ultimately cell death. The epit[r]

hampered by a lack o f adequate tools to evaluate not onlytotal cellular PtdIns, but also local c oncentrations within thecells. M eanwhile, u sing actin-binding drugs, we showed thatthe express ion of the CTGF/CCN2 gene can be modulatedby either actin polymerization or the availa bility of poly-mer[r]

in a Lab-Line incubator-shaker. More than 98% of theseglial cells stained positive for microglial marker Mac-1(CD11b/CD18; Boehringer Mannheim, Indianapolis, IN;data not shown). All animal protocols were approved bythe Committee of Animal Research at the University ofSouth Florida, in accorda[r]