EXPRESSION OF HUMAN VASOPRESSIN AND OXYTOCIN RECEPTORS IN ESCHERICHIA COLI

Various constructs with either N-terminal or smaller C-ter-minal extensions failed to produce any soluble fusion pro-tein. Furthermore, all five constructs produced Pfg27 thatprecipitated after protease cleavage from its fusion partner.The sixth construct, which produced soluble protein in hig[r]

synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii Pham Thi Tran Chau, M.K.Reddy, Dao Thi Thuy, Hoang Thu Ha, Le Nguyen Dung, Laurent Chiche 1. Introduction Proteinases are involved in different crucial living processe[r]

of 11-deoxycortisol into the major mammalian glucocorticoid, cortisol. Thereduction equivalents needed for this reaction are provided via a short elec-tron transfer chain consisting of a [2Fe-2S] ferredoxin and a FAD-contain-ing reductase. On the biochemical and biophysic[r]

receptorfrom striatal membranes obtained with Chaps/cholic acid,with Chaps and with digitonin [39±41]. Using our detergentcombinations, hA2aR was much more stable in solubilizedas well as puri®ed form than A2areceptor solubilized withChaps [ 40] that lost 45% of binding sites wi[r]

protein, OraSE-K629M, which is unable to bind P LP, wasconstructed and produced in E. coli. As the bacterial strainused to express protein is unable to synthesize cobalamin byitself, neither AdoCbl nor PLP could be involved in themutant protein folding process during i[r]

Hình 7: Ảnh hưởng của ion sodium trên hoạt tính của MalQ Hoạt tính của enzym MalQ không bị ưc chế bởi nồng độ ion sodium cao. Khi nồng độ sodium chloride được tăng lên từ 200 mM đến 500 mM trong dung dịch đệm 50 mM sodium acetate, hoạt tính của enzym này chỉ thay đổi 11% (Hình 6 và 7). 4 KẾT LUẬN Vớ[r]

Noronha, S.B., Yeh, H.J., Spande, T.F.& Shiloach, J. (2000). Investigation of the TCA cycle and the glyoxylate shunt in Escherichia coli BL21 and JM109 using (13)C-NMR/MS. Biotechnol Bioeng, Vol. 68, pp. 316-327 Parsell, D.A.& Sauer, R.T. (19[r]

and Wachsmuth I. K., 1987. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J. Infect. Dis. 156: 175 - 182. 3934. Leyer G. J., Wang L., and Johnso[r]

syndrome. Eur. J. Clin. Microbiol. Infect. Dis. 13:1074-1076. 49. Toma C., Lu Y., Higa N., Nakasone N., Chinen I., Baschkier A., Rivas M., and Iwanaga M., 2003. Multipex – PCR Assays for identification of human diarrheagenic Escherichia coli. J. Clin. Microbiol. 4[r]

as corepressors, may influence Bach1 activity.It is noteworthy that HO-1 expression is not reduced byhypoxia in th e two RPE cell lines, unlike other types ofhuman cells [27,28]. To confi rm these findings, we wish toperform experiments using primary cultures of human RPE,bu[r]

hydroxysteroid dehydrogenase type II enzyme, which normally inactivates cortisol and prevents it from binding to renal mineralocorticoid receptors. Consequently, in addition to the excess mineralocorticoids produced by ACTH stimulation of the adrenal gland, high levels