LOCALIZING FLEXIBLE REGIONS IN PROTEINS USING HYDROGEN DEUTERIUM EXCHANGE MASS SPECTROMETRY

November2009, accepted 23 November 2009)doi:10.1111/j.1742-4658.2009.07511.xThe translocation domain (T domain) of diphtheria toxin adopts a partiallyfolded state, the so-called molten globule state, to become functional atacidic pH. We compared, using hydrogen ⁄ deuterium ex[r]

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sites that have not been positively identified (data notshown; see discussion), as well as seven tyrosines. It haspreviously been shown that BPV E1 labelled in vivo with32P phosphate does not contain label on tyrosine, asshown by phosphoamino acid analysis [21,24]; thereforethe predicted tyro[r]

[Asn177(ND2)-Glu44(OE2)] occurring in middle of the helical bundle is also shown.S. Rajagopal and S. Vishveshwara Short hydrogen bonds in proteinsFEBS Journal 272 (2005) 1819–1832 ª 2005 FEBS 1829has 31 numbers (42 from hbplus) of SHBs and severalof them in crucial locati[r]

dependent on changes in calcium dynamics within the host cell, with increases leading to the detachment and subsequent availability of the substrate to participate in the bioluminescent reaction (Anderson et al., 1974). This system has been adapted, however, so that when the luciferase[r]

one unique peptide LTVHPTPMSED that did not matchany known protein in the nonredundant database. Adegenerate 5¢ primer designed to this peptide and a3¢ primer to peptide QAPVYFYE were synthesized(Fig. 4). These degenerate primers amplified a 1-kb DNAproduct from Sprague–Dawley rat liver cDNA.[r]

and the thioredoxin-based one.TPX activities monitored here can be considered physio-logical. Indeed, apparent Kmvalues for thioredoxin are of amicromolar range, compatible with in vivo levels. An in vivocompetition between GSH and thioredoxin for the plantGPXs cannot be ruled out, bec[r]

Characterization of Tin-catalyzed silicon nanowires synthesized by the hydrogenradical-assisted deposition methodMinsung Jeon⁎, Hisashi Uchiyama, Koichi KamisakoDepartment of Electronic and Information Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8[r]

Biol 1999, 294(5):1351-1362.29. Blom N, Sicheritz-Ponten T, Gupta R, Gammeltoft S, Brunak S: Pre-diction of post-translational glycosylation and phosphoryla-tion of proteins from the amino acid sequence. Proteomics2004, 4(6):1633-1649.30. Zanardi TA: Regulation of bovine papillomavirus DNA re[r]

44. Chernos VI, Vovk TS, Ivanova ON, Antonova TP, Loparev VN:[Insertion mutants of the vaccinia virus. The effect of inacti-vating E7R and D8L genes on the biological properties of thevirus]. Mol Gen Mikrobiol Virusol 1993, Mar-Apr(2):30-34.45. Broyles SS, Moss B: Identification of the vaccinia viru[r]

44. Chernos VI, Vovk TS, Ivanova ON, Antonova TP, Loparev VN:[Insertion mutants of the vaccinia virus. The effect of inacti-vating E7R and D8L genes on the biological properties of thevirus]. Mol Gen Mikrobiol Virusol 1993, Mar-Apr(2):30-34.45. Broyles SS, Moss B: Identification of the vaccinia viru[r]

– They are accelerated by a potential of 70 V– The electrons and molecules cross (usually at a right angle) and collide– The ions are primarly singly-charged, positive ions, that are extracted by a small potential (5V) through a slit See also Fig. 20-3, pg. 502 in Skoog, et al.Diagram from F[r]

(see Fig.14). Peaks in magnetic sector mass spectrometers usually are called separated to a 10% valley when the overlap point is at 1/10 of the height of the higher of the two peaks. If only one peak is available, resolution is determined by the quotient of the observed mass div[r]

show that both states are not significantly mixed. Thesharp resonance from Met20 (N7 & O6) is in bothcases very well resolved and appears at 118.1 p.p.m.(N) and 117.5 p.p.m. (O). Both lines are only0.5 p.p.m. wide and would be present simultaneously ifsamples contained a significant N ⁄[r]

was dose-dependent (Fig. 1A). Histological analysisrevealed morphological defects associated with the fat bodyappearing two to three days postinfection as cells started toshrink and stained more strongly with toluidin blue(Fig. 1B,D). Similar changes were detected in cells of theperioveolar e[r]

a normalizer differs too much in molecular mass fromthe protein of interest because it is exposed to differentgel ⁄ transfer inhomogenieties and therefore does notpermit an adequate estimation to be made of the blot-ting error. To ensure accuracy of data normalization,we applied spline[r]

Sơ lược lịch sử phát triển của phương pháp phổ khối lượngPhương pháp phổ khối lượng, viết tắt MS (Mass Spectrometry), là một phương pháp phân tích công cụ quan trọng để phân tích thành phần và cấu trúc của các hợp chất vô cơ và hữu cơ. Lịch sử phát triển của phương pháp được bắt đầu từ[r]

3Exact Mass: 150.03Mol. Wt.: 150.13m/e: 150.03 (100.0%), 151.04 (8.8%)C, 64.00; H, 4.03; O, 31.97Molecular MS Applications: Proteomics Proteome: The group of proteins related to a cell type (with a certain genome) under certain conditions (often forced on the cell) Genome: The comple[r]

of synaptic plasticity by binding to the adaptor proteinPSD95. This protein contains several PDZ domains oneof which binds to the few most C-terminal amino acidsof the NMDA receptor. This particular feature wasexploited for the retrieval of a PSD95-containing complexby immobilizing a hexapeptide cor[r]

diagnostic tool for early-stage cancer: a review of its progress to a clinically relevant tool. Mol Diagn 2004, 8:77-85.14. Posadas EM, Simpkins F, Liotta LA, MacDonald C, Kohn EC: Proteomic analysis for the early detection and rational treatment of cancer-realistic hope? Ann Oncol 2005, 16:16-22.15[r]

the M-F model, just do not apply in a properly constructed 2-country stock-flow model. External deficits or surpluses do not generate any change in the money supply, which remains entirely demand-determined, as the economists of the Banque de France used to argue through the compensati[r]