mature MARCKS mRNA and more weakly the 4.3 kbprecursor RNA containing an intron of 1.7 kb. RNAisolated from PCC7-Mz1 cultures treated with solvent orwith PDB (200 nM) for 5 h verified that the 2.6 kbMARCKS mRNA was not down-regulated in PCC7-Mz1cells by PDB treatment as was the case in[r]
sequences. Our results are consistent with studies of PABPfrom Xenopus [19], which showed the domain 1,2 pair tohave greater selectivity than the domain 3,4 pair forpoly(A) over other homopolymeric sequences, although inthis case affinity constants were not determined. That theselectivity has been ma[r]
envelope deletion mutant. J Virol 1993, 67:1854-9.76. Steinhauer DA, Domingo E, Holland JJ: Lack of evidence for proof-reading mechanisms associated with an RNA viruspolymerase. Gene 1992, 122:281-8.77. Su L, Kaneshima H, Bonyhadi ML, Lee R, Auten J, Wolf A, Du B, RabinL, Hahn BH, Terwilliger[r]
time-consuming than direct sequencing. Preliminary data reveal a 96% concordance at the genotype level and a 93% concordance on the genotype 1 subtype level when compared to direct sequencing of the NS5B and 5’UTR regions. Implications for Diagnosis and Management Diagnos[r]
CC:GG) were analyzed for hydrolytic cleavage. Further-more, the CU:AG motif was inverted to a nonreceptorsequence (GA:UC) and analyzed together with both thewild-type GCGA and the GUGA P9b tetra-loops. Whereasall the GNRA L9b tetra loops tested supported hydrolysiswell compared to UUCG[r]
3. Sellon RK: Update on molecular techniques for diagnostictesting of infectious disease. Vet Clin North Am Small Anim Pract2003, 33:677-693.4. Ivnitski D, O'Neil DJ, Gattuso A, Schlicht R, Calidonna M, Fisher R:Nucleic acid approaches for detection and identification ofbiological warf[r]
3. Sellon RK: Update on molecular techniques for diagnostictesting of infectious disease. Vet Clin North Am Small Anim Pract2003, 33:677-693.4. Ivnitski D, O'Neil DJ, Gattuso A, Schlicht R, Calidonna M, Fisher R:Nucleic acid approaches for detection and identification ofbiological warf[r]
Anionic liposomes, such as dioleoylphosphatidylserine(DOPS) were also used, and were more effective than theneutral or cationic liposomes for in vitro delivery. But allthese delivery vectors were all at a low efficiency level.Another RDO vector is polyethylenimine (PEI). PEI is apolymer with a[r]
of a new protein family with unique enzyme activity that has a putativerole in insect development. In addition, UDE may also serve as potentialtool in molecular biological applications. Owing to lack of homology withother proteins with known structure and ⁄ or function, de novo data arerequir[r]
RG62 ⁄ 07) and Nanyang Technological Universitystart-up grants (SUG5 ⁄ 06 and RG138 ⁄ 06) to ATP.References1 Moyzis RK, Buckingham JM, Cram LS, Dani M,Deaven LL, Jones MD, Meyne J, Ratliff RL & Wu JR(1988) A highly conserved repetitive DNA sequence,(TTAGGG)n, present at the tel[r]
là tin tức của cả dây DNA. Những tin tức di truyền này, vốn liên hệ đến sự sắp xếp của các amino acid (4), được biểu thị bởi những chuỗi ba gốc nitrogen, gọi là triplets (cặp ba; còn có tên làcodons), thí dụ như CGA, TTA, hay CGT,... Dựa vào tin tức liên hệ đến thứ tự của nhóm amino acid tương ứng,[r]
Page 5 of 7(page number not for citation purposes)based qRT-PCR suggest that there was a latent phase of atleast eight hours following initiation of infection, a periodduring which no significant increase in Nipah virus RNAsynthesis could be detected in the infected Vero cells. Arapid rise in the in[r]
-RNA (RNA tổng số hoặc chỉ mRNA) được phân tách bằng điện di trên gel agarose. Hình 3.1: Sơ đồ mô tả phương pháp Southern blot -RNA sau khi đã phân tách được chuyển lên màng lai (các phân tử RNA giữ nguyên vị trí như ở trên gel).-RNA cố định trên màng được lai với[r]
DNA is in a double strandThe nitrogen bases have compliment partnersAdenine-ThymineCytosine-GuanineJust a note about RNA-RNA is single-stranded and acts as a code for protein synthesis.RNA is still made of nucleotides that have a phosphate, a sugar, and a nitr[r]
carries out protein synthesis. This nucleic acid is called RNA, orribonucleic(ry boh noo klee ihk) acid. Three kinds of RNA = messengermRNA, Transfer tRNA, Ribosomal rRna.RNA contains ribose instead ofdeoxyribose and uracil instead of thymine % 4 5 N O Y Z t u Š ‹ Œ î[r]