PROTEIN EXPRESSION

Carbonic anhydrase related proteins (CARPs) VIII, X and XI functionally differ from the other carbonic anhydrase (CA) enzymes. Structurally, they lack the zinc binding residues, which are important for enzyme activity of classical CAs.

communication (GJIC) of cells.Method: In the present study, the effects of triphenyltin chloride (TPTC) on GJIC in WB-F344 rat liver epithelial cells were evaluated, using the scrape-loading dye transfer technique.Results: TPTC inhibited GJIC after a 30-min exposure in a concentration- and time-depe[r]

 According to Butler (2004a), contamination is one of reasons resulting the failure in operation of cell cultures. Growing at slow rate could make culture of animal cells be vulnerable to microbial contaminants (Butler, 2004a). Another factor resulting in the high rate of contamination in the cultu[r]

Aiyar, N., Baker, E., Wu, H. L., Nambi, P., Edwards, R. M., Trill, J. J., Ellis, C., andBergsma, D. J. 1994. Human AT1 receptor is a single copy gene: characteriza-tion in a stable cell line. Mol. Cell Biochem. 131:75–86.Allet, B., Bernard, A. R., Hochmann, A., Rohrbach, E., Graber, P., Magnenat, E.[r]

protein expression in gastrocnemius mitochondria. Wefound that fasting moderately increases (1.5-fold) or doesnot change UCP3 protein in gastrocnemius SS or IMFmitochondria, respectively. These results show that modu-lation of UCP3 expression at the mRNA level does notnec[r]

RECOMBINANT PROTEIN PRODUCTION: EXPRESSION SYSTEMS ANDANIMAL CELL TECHNOLOGYTÓM TẮTProtein tái tổ hợp đã và đang đƣợc áp dụng rộng rãi trong lĩnh vực y dƣợc nhằmphòng ngừa và điều trị bệnh. Trong bài báo này, tác giả đã đề cập đến một số dòng tếbào prokaryote và eukaryote hiện đang đƣợ[r]

data such as gene expression profiles, genotypes, serumprotein panels, metabolomics and proteomics data.Data is stored in an Oracle 11 database which is fullyauditable. We selected a set of open-source componentsto assemble the application in contrast to the strategyfollowed by Merck. A user[r]

Department of Surgery, University of Virginia Health System, Charlottesville, VA, USAEmail: Kimberley A Walsh - kim_walsh41@hotmail.com; Joseph F Megyesi - jmegyesi@uwo.ca; John X Wilson - john.wilson@fmd.uwo.ca; Jeff Crukley - j_crukley@hotmail.com; Victor E Laubach - vel8n@virginia.edu; Robert R H[r]

RESEARC H ARTIC LE Open AccessAlendronate (ALN) combined with Osteoprotegerin(OPG) significantly improves mechanical propertiesof long bone than the single use of ALN or OPG inthe ovariectomized ratsYan Wang1*, Peng Huang1, Pei-Fu Tang1, Kai-Ming Chan2,3and Gang Li2,3,4*AbstractBackground: Alendrona[r]

RESEARC H ARTIC LE Open AccessAlendronate (ALN) combined with Osteoprotegerin(OPG) significantly improves mechanical propertiesof long bone than the single use of ALN or OPG inthe ovariectomized ratsYan Wang1*, Peng Huang1, Pei-Fu Tang1, Kai-Ming Chan2,3and Gang Li2,3,4*AbstractBackground: Alendrona[r]

(osmoprotestants); trong vài trường hợp, chúng ổn định được các phân tử chức năng dưới điều kiện bị stress (Kishor và ctv. 1995; Hayashi và ctv. 1997; Shen và ctv. 1997; Garg và ctv. 2002). Protein LEA (late embryogenesis abundant) cũng được chú ý trong điều kiện bị stress do thiếu nước (Xu v[r]

types. That UCP2 was upregulated in aP2-Ucp1 mice wassomehow surprising and suggested that UCP1 and UCP2function differently in adipocytes. This supports the ideathat both UCP2 and UCP3 are linked to fatty acidoxidation [53] that is elevated by respiratory uncoupling inadipocytes [19]. It i s not cl[r]

cortex, hippocampus, and nucleus accumbens is altered in adult malemice exposed to repeated low dose of malathion. Synapse 2008,62(4):283-290.26. Savage E, Keefe T, Mounce L, Heaton R, Lewis J, Burcar P: Chronicneurological sequelae of acute organophosphate pesticide poisoning.Arch Environ Health 19[r]

Boyd, D., and Beckwith, J. 1990. The role of charged amino acids in the localiza-tion of secreted and membrane proteins. Cell 62:1031–1033.Buell, G., Schulz, M. F., Selzer, G., Chollet,A., Movva, N. R., Semon, D., Escanez,S., and Kawashima, E. 1985. Optimizing the expression in E. coli of a s[r]

Fig. 2. Presence of amyloid-like structures in the IBs formed by the prion protein HET-s from P. anserina. (A) HET-s PFD IBs from E. coliobserved by cryo-electron microscopy in intact E. coli cells. (B) Transmission electron micrograph of negatively stained purified HET-s PFDIBs. (C), (D) HET-[r]

well es tablished in g reen algae. I n the absence of oxygen,C. reinhardtii, a nd also plants, s witch their metabolism t ofermentation [43,44]. In the light, algae degrade cellularstarch via g lycolysis [45 ] and hydrog en gas is evolved. It hasbeen suggested that reducing equivalents from the glyc[r]

h repeat-containing G-protein-coupled receptor 5 (LGR5) is a promising intestinal stem cell and carcinoma stem cell marker. We examined the relationship between mismatch repair (MMR) protein deficiency and LGR5 expression in poorly differentiated (PD) colorectal carcinoma (CRC).

This study examined the prognostic significance of microtubule-associated protein light chain 3B (LC3B) expression in oropharyngeal and oral cavity squamous cell carcinoma (SCC). The prognostic significance of LC3B expression in relation to human papillomavirus (HPV) status in oropharyngeal SCC was[r]

NADPH: effect of various factors on the kinetics ofdegradation. Clin Chem 32, 314–319.14 Siebers B, Wendisch VF & Hensel R (1997) Carbohy-drate metabolism in Thermoproteus tenax: in vivoutilization of the non-phosphorylative Entner-Doudoroff pathway and characterization of its firstenzyme, gl[r]