BACULOVIRUS EXPRESSION SYSTEM

Fish recombinant gonadotropins byBerta LEVAVI-SIVAN (1), Matan GOLAN (1), Joseph AIZEN (1) & Abigail ELIZUR (2) ABSTRACT. - Pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play central roles in regulating gametogenesis and the production of gonadal ho[r]

metabolism [6,8,15]. Although the analyzed SCPLacyltransferases have maintained the nature andconfiguration of the Ser-His-Asp catalytic triad fromhydrolases, designed to perform nucleophilic cleavageof peptide or ester bonds, these enzymes have losthydrolytic activity towards peptide substrates [8].[r]

Pennell, C. A. 1997. High-level secretion of two antibody single chain Fv frag-ments by Pichia pastoris. J Immunolog. Meth. 201:67–75.Ellinger, S., Mach, M., Korn, K., and Jahn, G. 1991. Cleavage and purification ofprokaryotically expressed HIV gag and env fusion proteins for detection ofHIV antibodi[r]

when compared to constructs without these sequences;and suggested that expression of other caliciviruses (NoVand SaV) rVP1 that resulted in low yields or unstable VLPsmay be due to constructs that lacked the VP2 gene [6]. Analternative explanation was that point mutationsinfluenced steady-sta[r]

tein; surface display.The baculovirus Autographa californica nuclear polyhedro-sis virus has been widely used as an expression system foreukaryotic proteins in insect cell culture [1,2], for surfacedisplay of various peptides and proteins [3,4], and morerecently, for the transdu[r]

identification and characterization of cytosolic sulfotrans-ferases (STs) in zebrafish. By searching the zebrafishexpressed sequence tag database, we have identified twocDNA clones encoding putative cytosolic STs. These twozebrafish ST cDNAs were isolated and subjected to nuc-leotide sequencing. Sequence[r]

found this residue was strictly conserved, but more impor-tantly, there was a strictly conserved amino acid motif atthis site, NGDV (data not shown). However, when weincluded a porcine SaV GIII strain and a recently identi-fied SaV GIV strain (PEC and Hou-7, respectively), onlythe GD site was strict[r]

19particularly interested in the immunomodulatory roles of several bacterial heat shockproteins, namely Burkholderia pseudomallei Hsp70, B. pseudomallei Hsp60 andMycobacterium bovis Hsp65. It is fascinating to examine whether their ability tocross-present is comparable and to look at their immunolog[r]

tion may, in part, result in the seeking of additional fund-ing, public or private, to support follow-up studiespertinent to the work outlined herein. Luis M Branco, AlexMatschiner, Darryl B Sampey, Joseph N Fair, Robert FGarry, and Mary C Guttieri are listed inventors, in addi-tion to others, in a[r]

51. Himeda T, Ohara Y, Asakura K, Kontani Y, Murakami M, Suzuki H,Sawada M: A lentiviral expression system demonstrates thatL* protein of Theiler's murine encephalomyelitis virus(TMEV) is essential for virus growth in a murine macro-phage-like cell line. Virus Res 2005, 108:23-28.52. v[r]

were constructed with a baculovirus expression system and the subsequentenzyme activity of sku-type BmIVD was shown to be significantly reducedcompared with that of wild-type BmIVD. Molecular modelling suggestedthat this reduction in the enzyme activity may be due to negative eff[r]

BioMed CentralPage 1 of 9(page number not for citation purposes)Virology JournalOpen AccessResearchExpression and processing of the Hepatitis E virus ORF1 nonstructural polyproteinDeepak Sehgal, Saijo Thomas, Mahua Chakraborty and Shahid Jameel*Address: Virology Group, International Center for Genet[r]

correlation functions, the time-dependent spin autocorre-lation function is closely linked with spin dynamics,therefore, it is natural to focus on this quantity. Earlierstudies have numerically investigated the time-dependentspin autocorrelation function of many-spin systems such asa classical Heise[r]

Vào những năm 70 bệnh virus trên tôm lần đầu tiên được phát hiện bởi Couch 1974 và gọi là _Baculovirus penaeid_, và hiện nay con người đã tìm ra khoảng 20 bệnh do virus trên tôm và hàng [r]

tive regulator of SAF-1 function under normal condi-tions [23]. During inflammation, SAF-2 expression isdown-regulated, which alleviates the repression ofSAF-1 activity and further promotes SAF-1-mediatedtransactivation of the target genes [23]. In this paper,we present evidence for a third me[r]

and IL-6. These cytokines appear to be responsible for the neurotoxicityobserved in peripheral nervous system inflammatory disease. It has beenreported that, in the central nervous system, the expression level of inter-cellular adhesion molecule-1 (ICAM-1) was dramatically upregu[r]

drome virus. Virology 2002, 301:136-147.12. van Hulten MCW, Westernberg M, Goodall SD, Vlak JM: Identifica-tion of two major virion protein genes of white spot syn-drome virus of shrimp. Virology 2000, 266:227-236.13. Halford WP, Kemp CD, Isler JA, Davido DJ, Schaffer PA: ICP0,ICP4, or VP16 expresse[r]

levelofintratumoralchemotherapy can be achieved by conversion of prodrug, which is aremarkable benefit of the suicide gene/prodrug system.91.3.1 Herpes Simplex Virus Type 1 (HSV-1) ThymidineKinase(HSVtk)/Ganciclovir(GCV)Moolten (1986) first reported the HSVtk/GCV system. Nontoxic GCV i[r]

BioMed CentralPage 1 of 7(page number not for citation purposes)Virology JournalOpen AccessResearchBaculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 geneLiqun Lu, Hai Wang, Ivanus Manopo, Li Yu and Jimmy Kwang*Address: Animal health biotechnology un[r]

Instead of using ANN based on the large image representation space, ANN is used to classify Facial Expression based on PCA representation. So the training time of ANN is reduced. To experience the feasibility of our approach, in this research, we built recognition of six basic facial expressi[r]