QUANTITATIVE PROTEOME AND PHOSPHOPROTEOME ANALYSIS OF HUMAN PLURIPOTENT STEM CELLS

formation of embryoid bodies, and analyse their ability to give rise tohematopoietic precursors, with emphasis on their engraftment potential asa measure of their functionality in vivo.AbbreviationsAGM, aorta–gonad–mesonephros; BFU-E, burst forming unit-erythrocyte; BMP4, Bone m[r]

54. Tateishi K, He J, Taranova O, Liang G, D’Alessio AC, Zhang Y: Generation ofinsulin-secreting islet-like clusters from human skin fibroblasts. J BiolChem 2008, 283:31601-7.55. Dimos JT, Rodolfa KT, Niakan KK, Weisenthal LM, Mitsumoto H, Chung W,Croft GF, Saphier G, Leibe l R, Golan d R, Wi[r]

lineage restrictions (called transdifferentiation). For example, HS cells may be converted into neurons as well as germ cells. This feature may provide a means to use tissue stem cells derived directly from a patient for therapeutic purposes, thereby eliminating the need[r]

Bone marrow for leukemia & chemotherapy: Tủy xương sản xuất bạchcầu và ứng dụng trong hóa học trị liệuNerve Cells for Parkinsons & Alzhiemer’s disease: Tế bào thần kinh ứngdụng trong điều trị chứng mất trí và bệnh ParkinsonHeart Muscle Cells for heart disease: Tế bào cơ[r]

ES cells has been controversial, and their use in clinical applications would be unacceptable to some patients and physicians despite their enormous potential. Somatic cell nuclear transfer ("therapeutic cloning") represents an alternative method for creating ES cell lines that[r]

Commun 1999, 259(3):523-526.7. Radonic A, Thulke S, Bae HG, Muller MA, Siegert W, Nitsche A: Ref-erence gene selection for quantitative real-time PCR analy-sis in virus infected cells: SARS corona virus, Yellow fevervirus, Human Herpesvirus-6, Camelpox virus and Cytomeg-a[r]

ducted data analysis with the help of SM. AC providedintellectual input, and grant funding. All authors read andapproved the final manuscript.AcknowledgementsWe would like to thank the following members of the Centre for Virus Research for providing infected cell extracts[r]

Chapter 066. Stem Cell Biology (Part 2) Self-Renewal and Proliferation of Stem Cells Symmetric and Asymmetric Cell Division The most widely accepted stem cell definition is a cell with a unique capacity to produce unaltered daughter cells (sel[r]

J. Immunol. Meth. 65, 55–63.22. Doyle, A., Griffiths, J.B. & Newell, D.G. (1995) Cell and TissueCulture: Laboratory Procedures. John Wiley & sons, New York,USA.23. Vermes,I.,Haanen,C.,Steffens-Nakken,H.&Reutelingsperger,C. (1995) A novel assay for apoptosis. Flow cytometr[r]

Page 11 of 12through a STAT3 pathway in cholangiocarcinoma cells. Hepatology 2005,42:1329-38.20. Yu J, Ryan DG, Getsios S, Oliveira-Fernandes M, Fatima A, Lavker RM:MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels inepithelia. Proc Natl Acad Sci USA 2008, 105:19300-5.21.[r]

on the cell membrane. Seya et al. [16] showed a decreaseof CD46 expression on leukaemia cell lines by in vitro G-CSF treatment.Summing up, our results show significant expression ofCD46 on various types of blood leukocytes includinghematopoietic progenitor cells. Consequently these cel[r]

tumor samples and paired surrounding non-cancerous tissue obtained endoscopically, the association withhistopathological differentiation was examined with quantitative RT-PCR.Results: Based on the miR microarray analysis, there were 14 miRs that showed significant differences (m[r]

Glioma stem cells (GSCs) contribute to tumor recurrence and drug resistance. This study characterizes the tumorigenesis of CD133+ cells and their sensitivity to pharmacological inhibition.

Adams V:Time-dependent mobilization of circulating progenitor cellsduring strenuous exercise in healthy individuals. J Appl Physiol 2009,107(6):1943-50.27. Kmieciak M, Gowda M, Graham L, Godder K, Bear HD, Marincola FM,Manjili MH: Human T cells express CD25 and Foxp3 upon[r]

C, De MR: Identification and expansion of human colon-can-cer-initiating cells. Nature 2007, 445:111-115.23. Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkel-man RM, Cusimano MD, Dirks PB: Identification of human braintumour initiating [r]

41(6):1025-1035.80. Wang E, Miller LD, Ohnmacht GA, Liu ET, Marincola FM: High-fidel-ity mRNA amplification for gene profiling. Nat Biotechnol 2000,18(4):457-459.81. Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S,Ellis B, Gautier L, Ge Y, Gentry J, et al.: Bioconductor: open soft-[r]

fiction, disease, cure, tissue, organs,embryo, foetus, abort, kidney/heart failure,cells, transplant.2 Ask students to work in pairs. Handout the top part of the worksheet. Studentsread the introductory text. To checkcomprehension, ask students what cloningis and is not, accordin[r]

To explore the regulation of human Cdc45 in nonpro-liferating cells in more detail, the differentiation ofhuman cells was used as a second system. HumanCdc6 protein was completely downregulated duringin vitro differentiation of K562 cells to cells wit[r]

cells were compared with HbE/b-thalassemia and normal HSCs.Results: A significant change in abundance of 229 phosphoproteins was demonstrated. Importantly, the analysis ofthe candidate proteins revealed a high abundance of proteins that are commonly found in apopto[r]

Piangitjagum A, Wasi P, Ma L, Schrier SL: A correlation of erythrokinetics,ineffective erythropoiesis, and erythroid precursor apoptosis in thaipatients with thalassemia. Blood 2000, 96(7):2606-2612.8. Kong Y, Zhou S, Kihm AJ, Katein AM, Yu X, Gell DA, Mackay JP, Adachi K,Foster-Brown[r]