DETECTION OF HERPES SIMPLEX VIRUS IN CSF BY PCR

methods for detecting HSV tend to be slow and insensitive. We designed a rapid PCR-based assayto quantify and type HSV in cervicovaginal lavage (CVL) fluid of subjects attending a Genito-UrinaryMedicine (GUM) clinic. Vaginal swabs, CVL fluid and venous blood were collected. Quan[r]

(reviewed in [13]). It acts to enhance mRNA accumula-tion, at least in part by stimulating transcription [14,15].However it does not bind DNA and there is no evidencethat it acts directly on the transcriptional apparatus.Rather, ICP0 appears to stimulate HSV gene expression atle[r]

65-day observation period with no evidence of viral infection although the immunocompetent micedeveloped cellular and humoral immunity to HSV-1. In contrast, BALB/c scid recipients of gangliacontaining reactivating virus invariably developed a local and, subsequently, sys[r]

Enteropathogenic Escherichia coli : characterization of a New Intimin Variant. Infection and Immunity. 68: 64 - 71. 41. Paton A. W., Ratcliff R., Doyle R. M., Seymour-Murray J., Davos D., Lanser J. A., and Paton J. C., 1996. Molecular microbiological investigation of an outbreak of[r]

(B) Confirmation of HSV-1 entry into RPE cells by X-gal staining. RPE cells grown (4 · 106cells) in six-well dishes were challenged withb-galactosidase-expressing recombinant HSV-1 (gL86) at 20 PFU per cell. Wild-type CHO-K1 cells and nectin-1-expressing CHO-K1 cells werealso inf[r]

epidermal cells after axonal transmission. J Virol 2001,75:11821-11826.25. Blatt AN, Laycock KA, Brady RH, Traynor P, Krogstad DJ, Pepose JS:Prophylactic acyclovir effectively reduces herpes simplexvirus type 1 reactivation after exposure of latently infectedmice to ultraviolet B. Inve[r]

(reviewed in [13]). It acts to enhance mRNA accumula-tion, at least in part by stimulating transcription [14,15].However it does not bind DNA and there is no evidencethat it acts directly on the transcriptional apparatus.Rather, ICP0 appears to stimulate HSV gene expression atle[r]

Survey of Sathgudi sweet orange was done in the Nagri village of Chittor district in the A.P. state during November, 2006. Incidence of citrus yellow mosaic disease was observed up to 50% in citrus plants. A few plants also showed unusual symptoms of vein clearing and mosaic. Bacilliform and flexous[r]

immune responses of microglia and astrocytes to DNAvirus infection and may represent an important compo-nent in the inflammation and damage associated withviral encephalitis.DiscussionSeveral members of the herpesvirus family includinghuman herpesvirus 6, HSV-1, and HSV-2 can el[r]

cells, suggesting a possible role for B5 in HSV-1 entry.Because gH also contains an a-helix, it is speculatedthat it may be a ligand for B5 [39]. No direct interac-tion between B5 and gH has been demonstrated todate. By contrast, in a separate study, a soluble formof gH-gL heter[r]

* Correspondence: loken006@umn.eduNeuroimmunology Laboratory, Center for Infectious Diseases andMicrobiology Translational Research, Department of Medicine, University ofMinnesota, Minneapolis, MN, USAHu et al. Journal of Neuroinflammation 2011, 8:123http://www.jneuroinflammation.com/c[r]

Authors’ contributionsJD carried out the serological and biological characterizations. JN carried outthe molecular characterizations of PVM isolates. HX designed andcoordinated the study and carried out the genetic analysis. All authors readand approved the final manuscript.Competing interest[r]

Chapter 043. Jaundice (Part 6) Hepatocellular Conditions Hepatocellular diseases that can cause jaundice include viral hepatitis, drug or environmental toxicity, alcohol, and end-stage cirrhosis from any cause (Table 43-2). Wilson's disease, once believed to occur primarily in young adults,[r]

dynein chains that interact with viral proteins. The importance of thesedynein–virus interactions has been supported, in principle, by the observa-tion that in some cases the dynein-interacting motifs of viral proteinsaltered by site-directed mutagene[r]

the Jackson Laboratories (Bar Harbor, ME). Purifiedmicroglial cell cultures (>99% pure), as determined byMAC-1 antibody staining (Roche Applied Science, Indian-apolis, IN), were prepared from these mice using a previ-ously described method with minor modifications [13].Growth medium fo[r]

Citrus Yellow Mosaic Virus (CYMV) disease and citrus greening bacterium are two important diseases in citrus. Citrus yellow mosaic virus (CYMV) disease is caused by bacilliform DNA virus while citrus greening disease caused by a fastidious bacterium (Candidatus liberibacter asiaticus) (Cla). Both th[r]

reports have shown that the expression of these genes canbe variable under several experimental conditions, mak-ing them inappropriate for use as normalisers [4-7]. Inreality no cellular gene maintains constant expression lev-els under all conditions and the evaluation of an appro-pria[r]

Commun 1999, 259(3):523-526.7. Radonic A, Thulke S, Bae HG, Muller MA, Siegert W, Nitsche A: Ref-erence gene selection for quantitative real-time PCR analy-sis in virus infected cells: SARS corona virus, Yellow fevervirus, Human Herpesvirus-6, Camelpox virus and Cy[r]

Several commercially available HSV-type specific serolog-ical assays are available, but a test such as a HSV-2 Westernblot is expensive and restricted largely to reference labora-tories. The Kalon test, which was found to be the bestamong a set of serological tests evaluated in samples[r]

trong tam cá nguyệt thứ ba với nam giới có herpes sinh dục II - Chẩn đoán  Chỉ dẫn để thử nghiệm - tổn thương loét, viêm não xác, với các triệu chứng viêm não đầu mối thần kinh, loét giác mạc o Phát hiện sớm bệnh là rất quan trọng kể từ khi điều trị với antivirals đáng kể có thể thay đổi khó[r]