RECOMBINANT PROTEIN

Recombinant proteins were developed more than 25 years ago. A large number of them are used as active pharmaceutical ingredients (Gnoth et al., 2008a). Those pharmaceutical proteins have been produced using several expression systems, such as bacteria (E. coli), filamentous fungi and yeast (S[r]

temperature (Tt) are solubilized and, above it, the chainsaggregate into larger structures. This behaviour was usedduring the purification process in order to retrieve thepurified polymer by cold and warm centrifugation cycles.The designed biopolymer has a theoretical molecularweight of 31,877 Da. The[r]

The H1N1 Hemagglutinin protein is known to carry neutralizing epitopes, hence the yeast derived glycosylated HA protein obtained from our study may find dual applications in both disease diagnostics and prophylaxis. As the main objective of the present study was confined to the reco[r]

In this study, a novel missense mutation in F11 wasidentified in a proband with mild type II FXI defi-ciency. In vitro expression of the FXI–Val371Ilerecombinant protein, followed by activation assays,showed slight differences in both FXI activation andFIX activation by thrombin-activated FXI.[r]

Noronha, S.B., Yeh, H.J., Spande, T.F.& Shiloach, J. (2000). Investigation of the TCA cycle and the glyoxylate shunt in Escherichia coli BL21 and JM109 using (13)C-NMR/MS. Biotechnol Bioeng, Vol. 68, pp. 316-327 Parsell, D.A.& Sauer, R.T. (1989). Induction of a heat shock-like respon[r]

knowledge of protein–protein interactions, we could selectareas potentially involved in the binding of protein S. Wewere interested in mutating residues close to the CCP1–CCP2 interface and amino acids of hydrophobic nature.Yet, it was also important to assess the role of charge[r]

filaments even when such additives were present (datanot shown). The results of the sedimentation equili-brium experiment indicate that the largest oligomerformed by the recombinant protein is approximately a30-mer. According to the model derived from electronmicroscopy, an axial rise f[r]

This could be achieved by different methods including frozen-thaw, ultrasonication, lysozyme treatment, or the combination of them. Depending on the forms of expressed protein, the target protein can be found in supernatant or precipitate of the cell lysate. This could be identified ea[r]

receptor, thereby relieving repression and allowing cells to differentiate and ultimately undergo apoptosis. This mechanism has therapeutic importance as the addition of retinoic acid to treatment regimens induces a higher remission rate in patients with promyelocytic leukemia (Chap. 104). Methylati[r]

protein based subunit vaccines offer an alternative over conventional vaccine strategies that could save several months of manufacturing time, since the HA gene of the newly circulating strain is available shortly after virus isolation or nucleotide sequencing of HA gene. In contrast to conve[r]

or to a lytic infection with recombinant baculovirus infected cells.General descriptions for the creation of stable insect cell lines are given by McCarroll and King (1997), Ivey-Hoyle (1991),and Benting et al. (2000). Invitrogen and Novagen sell reagents to produce such lines and provide det[r]

KeywordsHippocampus comes; C-type lectin; cDNAlibrary; male pregnancyCorrespondenceP. Melamed, Department BiologicalSciences, National University of Singapore,14 Science Drive 4, Singapore 117542Fax: +65 6872 2013Tel: +65 6874 1882E-mail: dbsmp@nus.edu.sg(Received 23 November 2004, revised 26Decembe[r]

analysis (see Experimental procedures) to derive the kinetic param-eters listed in [12]. (A) APC variants R9H (s); E16D (n); E26K (e);QGNSEDY (j). (B) Q32A (h); V34A (n); D35A (s). wildtype APC(r) and FVa only (d) are the same in both panels. Results areexpressed as the mean ± SD of three individual[r]

processing cells. Nonetheless, nonliving parenteral vaccines can be extremely efficacious. For example, hepatitis A vaccine appears to be effective in nearly 100% of recipients. Currently available nonliving vaccines consist of inactivated whole organisms (e.g., plague vaccine), detoxified protei[r]

February 2009, accepted 13 February 2009)doi:10.1111/j.1742-4658.2009.06968.xA survey of plasmids for 51 prion protein constructs from bank vole, cat,cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle,and wallaby, and for 113 mouse prion protein constructs[r]

homodimer displays an intrinsic specific GTPase activ-ity, whereas the monomer form remains completelyinactive. In terms of kinetics, the sigmoidal curve ofV0versus [GTP] is indicative of a co-operative bindingand hydrolysis model, and the Hill coefficient (h)of2.13 implies a strongly positive co-oper[r]

To determine the substrate specificity of 2,5-DKCMO avariety of compounds representing diffe rent classes ofBVMO-substrates were investigated in biocatalysisexperiments using the crud e enzyme extract (Figure 5).Only bicyclic ketones were converted under the chosenconditions by the crude extract con[r]

G93A-SOD1 spinal cords and the 5LOX antagonist nordi-hydroguaiaretic acid (NDGA) slows disease progressionin the ALS mouse [14]. These findings suggest a robust,multi-faceted neuroinflammatory response, antagonismof which may slow the progression of ALS.In order to better understand the contribution[r]

respectively.2080 T. Kitazume et al. (Eur. J. Biochem. 269) Ó FEBS 2002substrates and Arg47 and Tyr51 residues of the protein [30–32]. The aliphatic head of fatty acids would then turn andpenetrate the access channel. The more hydrophobicenvironment around the entrance of P450foxy would permi[r]

BioMed CentralPage 1 of 19(page number not for citation purposes)Virology JournalOpen AccessResearchInvolvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinantCarmen E Gómez, Andrée Marie Vandermeeren,[r]