HUMAN EMBRYONIC STEM CELLS FROM LABORATORY AND CLINICAL PERSPECTIVES

This study was aimed at comparing the in vivo osteogenic differentiationpotential of human embryonic stem cells (hESC) and human somatic osteoblast cellline.MethodHESCs were propagated on mouse embryonic feeder cells. They were shownto be pluri[r]

consensus 7-mer seed sequence and their targeted genes had overlapping functions. Among thetargeted genes, genes with chromatin structure modification function are enriched suggesting a rolein the maintenance of chromatin structure. We also found that the expression level of members ofthe two[r]

3Salk Institute, Department of Stem Cells, La Jolla, CA 92093, USA and 4Departments of Surgery, Microbiology/Immunology, Pathology, University of Western Ontario, London, Ontario, N6A 5A5, CanadaEmail: Grzegorz Wladyslaw Basak - gbasak@ib.amwaw.edu.pl; Satoshi Yasukawa - yasukaw[r]

ply of these angiogenic factors [5]. Bone marrow mono-nuclear cells containing small number of EPCsparticipated in neovascularization after focal cerebralischemia in mice [4] or patients with limb ischemia [19].However, Rehamn et al. demonstrated that EPCs, whichwere positive for acLDL and[r]

54. Tateishi K, He J, Taranova O, Liang G, D’Alessio AC, Zhang Y: Generation ofinsulin-secreting islet-like clusters from human skin fibroblasts. J BiolChem 2008, 283:31601-7.55. Dimos JT, Rodolfa KT, Niakan KK, Weisenthal LM, Mitsumoto H, Chung W,Croft GF, Saphier G, Leibe l R, Golan[r]

, vols 1 and 2. London, Elsevier Academic Press, 2004 Marshak DR et al (eds): Stem Cell Biology, New York, Cold Spring Harbor Laboratory Press, 2001 Odorico J et al (eds): Human Embryonic Stem Cells. New York, BIOS Scientific Publishers, 2005

embryos, and lowerthan wild-type numbers are found in ALK2 heterozy-gous animals. The same effect is true for BMP4 knock-outs and heterozygotes, which can then be rescued byectopic expression of a constantly active variant ofALK2. How these different BMP signals are finallyintegrated to[r]

At present, many types of primary cancer stem cellswith specific surface mark ers have been isolated and thecancer stem cell hypothesis is widely accepted. However,many questions remain in the field of cancer stem cellsresearch. For example, where primary cancer stem