IN VIVO AND IN VITRO APPLICATIONS TO THE STUDY OF ENZYM

three times for each cell line). All three cell lines sustained productive infection (Figure 1B, D and 1F). In order to analyze the CPE induced by BoHV-4EGFP Δ TK, cells were fixed with methanol and stained with Wright’s stain. A total of 600 cells were co[r]

The polyether antibiotic Salinomycin (Sal) is regarded as an inhibitor of cancer stem cells. Its effectiveness on human colorectal cancer (CRC) cells in vitro has been demonstrated before. The aim of this study was to establish a murine model to investigate the effectiveness of Sal in vivo. Furtherm[r]

Optimizing the safety and efficacy of standard chemotherapeutic agents such as cisplatin (CDDP) is of clinical relevance. Serum starvation in vitro and short-term food starvation in vivo both stress cells by the sudden depletion of paracrine growth stimulation.

The present work aims to study the in vitro properties of nano-hydroxyapatite/chitosan–gelatin composite materials. In vitro behavior was performed in simulated body fluid (SBF) to verify the formation of apatite layer onto the composite surfaces. The in vitro data proved the deposition of calcium a[r]

In this study, a number of articles on the fMRI images were reviewed, and some of the applications of these images were presented. Functional areas of fMRI images are the detection of brain activities and brain tumors, and brain mapping. Reviewing the valuable, albeit limited, papers undertaken on t[r]

Former studies already revealed the anti-neoplastic properties of the anti-infective agent Taurolidine (TRD) against many tumor species in vitro and in vivo. Its anti-proliferative and cell death inducing capacity is largely due to its main derivative Taurultam (TRLT).

In the present study, an in vitro tumor tissue model was constructed using alginate hydrogel, and in vitro cytotoxic efficacy of two anticancer drugs cisplatin and DZNep was investigated[r]

Identification of new drugs against paediatric sarcomas represents an urgent clinical need that mainly relies on public investments due to the rarity of these diseases. In this paper we evaluated the in vitro and in vivo efficacy of a new maltol derived molecule (maltonis), belonging to the family o[r]

Cholangiocarcinoma is characterized by late diagnosis and a poor survival rate. MicroRNAs have been involved in the pathogenesis of different cancer types, including cholangiocarcinoma. Our aim was to identify novel microRNAs regulating cholangiocarcinoma cell growth in vitro and in vivo.

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth an[r]

This work demonstrated that three-quarters of asth- matic persons living in Jeddah, Saudi Arabia, who were referred to the allergy clinic at King Abdulaziz University Hospital were sensitized to common indoor inhalant allergens. Additionally, there was a clear asso[r]

The purpose of this study is to i) fabricate a biodegradable nanoparticle formulation of Ketoprofen, ii) evaluate its characteristics, iii) investigate its in vitro dissolution and in vivo pharmaceutical property. The nanoparticle formulation was prepared by spray drying method using Eudragit L100 a[r]

In vitro and in vivo studies have suggested that osteopontin (OPN) is associated with many types of cancers. However, no studies have reported the incidence of OPN polymorphisms and the risk of gastric cancer.

Natural products from plants have been proven to be important resources of antitumor agents. In this study, we exploited the antitumor activity of (E)-3-(4-chlorophenyl)-N-(7-hydroxy-6-methoxy-2-oxo-2Hchromen-3-yl) acrylamide (SC-III3), a newly synthesized derivative of scopoletin, by in vitro and i[r]

Bio control agents (Trichoderma viride, Pseudomonas fluorescens) and various fungitoxicants were tested for their efficacy in controlling the Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceri in vitro and in vivo. In vivo conditions soil inoculated with Fusarium oxysporum f. sp. c[r]

Vandetanib (Caprelsa tablets, VNT) is an orally inhibitor of vascular endothelial growth factor receptor 2. The current research reports the characterization and identification of in vitro, in vivo and reactive intermediates of VNT. In vitro metabolites of VNT were performed by incubation with rat l[r]

In contrast to the tangible and obvious effective-ness of RNAi in vitro, silencing target gene expression in vivo using small interfering RNA siRNA has been a very challenging task due t[r]

Conclusions
Our data show that the CAM is an excellent in vivo model for NHL research, but has until recently been underestimated with regards to its similarities with pri- mary human lymphoma. We would like to stress that, depending on the scientific questions,[r]

dative damage is prevented by vitamin C, the subsequent physiological events, including lung damage, is pre- vented. Reports from other laboratories indicated that that it took at least four months of CS exposure to get appreciable lung damage in guinea pigs [13]. One r[r]

It is important to simultaneously induce strong cell death and antitumor immunity in cancer patients for successful cancer treatment. Here, we investigated the cytotoxic and phenotypic modulation effects of the combination of ANT2 shRNA and human sodium iodide symporter (hNIS) radioiodine gene thera[r]