AN UNDERGRADUATE EXPERIMENT USING CYCLODEXTRIN – ASSISTED SENSITIVE FLUORESCENCE DETECTION AND QUANTITATION OF DAPSONE DRUG IN WASTEWATER SAMPLES
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Annexin V-FITC showed enhanced apoptosis by VEVDMs To measure and compare the extent of apoptosis induced by 1 μ M of free DOX and VEVDMs on incuba- tion for 24 h, FITC-conjugated annexin staining was done and analyzed using flow cytometer. Annexin stain-[r]
Samples for histology were available in 26 patients. Of these, 13 fullfilled the EORTC/MSG criteria for host risk factors. Ten patients underwent pulmonary biopsy. Seven of these met the criteria of probable aspergillosis prior to biopsy, and could be reclas[r]
cules [15]. GdmCl-induced denaturation of the tryptophan mutants was monitored by the change in fluorescence emission spectra at an excitation wave- length of 295 nm. The results were plotted by taking the average emission wavelength [23] and the fluores- cence int[r]
This letter reports on mid-infrared laser-based detection and analysis of chemical species. Emphasis is put on broadly tunable laser sources and sensitive detection schemes. Selected examples from our lab illustrate the performance and potential of such systems in various areas including environment[r]
Recently, a loop-mediated isothermal amplification (LAMP) reaction was developed as an alternative method to meet the abovementioned requirements. The LAMP method allows the whole reaction process, including dena- turing, to proceed at a constant temperature by incubating the reagents in[r]
This study investigated the impact of using smartphones on the academic performance of undergraduate students at the North-West University, South Africa. To determine the impact, this study deployed the quantitative research approach to collect data from 375 undergraduate students using questionnair[r]
The objective of the study is to reveal the seasonal variations in the wastewater quality with respect to heavy metal contamination. To get the extent of the heavy metals contamination, wastewater samples were collected from 5 different locations from in and around river Narmada during the Winter, S[r]
derived from PVM RNAs. A single MscI (TGG ↓ CCA) site was confirmed in the region flanked by primer PVM3 and PVM4 in the CP gene of all the Canadian PVM isolates sequenced in this study and all other known PVM isolates except those PVM strains/isolates (M5[r]
Problems of this kind do not arise in baseball, but they have considerable practical importance in other realms. The impetus for the studies described in this chapter was the Strategic Defense Initiative (SDI), the plan conceived in the early 1980s for defending th[r]
In this paper, Origin is applyed to analyze samples and give some results about the fluorescence spectrum of SiO2-Al2O3 glass material, which is produced by Sol-Gel method.
TRANG 6 2158 TABLE.1 Detection of _Mycobacterium avium subspecies paratuberculosis_ antibodies in cow milk samples from organized and unorganized farms at Mhow of Indore in Madhya Prades[r]
In addition to genetic causes such as PAI 1-4G/5G already described, mutations in either the BRCA1 or BRCA2 genes accounted for 90 to 95% of familial breast cancer cases, which makes up for one quarter of breast cancer cases amongst women aged 30 years [r]
Unlicensed and off label drug use is an issue recognized worldwide in pediatric pharmacotherapy. The study was designed to assess the prevalence and predictors of unlicensed and off label drug use in neonatal population of Pakistan.
The conventional QA architecture has a drawback in that the question-type system restricts the range of questions that can be answered by the system. It is thus problematic for QA system developers to care- fully design and build an answer candidate extrac- tor that works[r]
In vitro cell proliferation test Human CRC HCT-116, LoVo, HT-29, and SW620 cells in logarithmic growth phases were plated at 5 × 10 3 cells/well in 96-well plates; the next day, culture media was replaced with 200 μ L culture medium containing VB (purity >98%, purc[r]
Detection of viral antigen by direct fluorescent antibody test (FAT) from clinical samples collected from cattle and buffaloes of Navsari, Surat and Valsad Districts of Southern Gujarat were carried out. Out of a total of 116 clinical samples (44-cattle, 72-buffaloes) tested, fluorescence was observ[r]
In Vietnam, nasopharyngeal carcinoma (NPC) is the eighth most common cause of death from cancer. Cell-free Epstein Barr virus DNA (cf-EBV DNA) was reported to be present in almost all NPC patients. However, currently available assays in Vietnam can detect cf-EBV DNA in only 67.6% of NPC patients, th[r]
The menace of Tuberculosis is age old. Concerns are mounting over the rise in the number of TB cases and more worryingly, drug resistant TB cases. Despite the fact that highly effective drugs are available today, the problem o[r]
methylation usually results in gene repression, so methylation permanently blocks XIST expression and permits the continued expression of all the other X-linked genes (Gartler et al., 1983). However, some genes on the X chromosome escape inactivation. These are present on[r]
Prompt detection of PRRSV in the field samples is important for effective PRRS control, thereby reducing the potentially serious economic damage which can result from an outbreak. Therefore, rapid, specific and sensitive assays are required for diag[r]