VALIDATION OF COL11A1/PROCOLLAGEN 11A1 EXPRESSION IN TGF-Β1-ACTIVATED IMMORTALISED HUMAN MESENCHYMAL CELLS AND IN STROMAL CELLS OF HUMAN COLON ADENOCARCINOMA
Tìm thấy 10,000 tài liệu liên quan tới tiêu đề "Validation of COL11A1/procollagen 11A1 expression in TGF-β1-activated immortalised human mesenchymal...":
The human COL11A1 gene has been shown to be up-regulated in stromal cells of colorectal tumours, but, so far, the immunodetection of procollagen 11A1, the primary protein product of COL11A1, has not been studied in detail in human colon adenocarcinomas.
an anti-inflammatory cytokine. Although in healthyfish IL-11 is expressed at very low levels in liver, headkidney and spleen, as evidenced from the high cyclenumber of 32 needed for its detection by RT–PCR, itsexpression is highly induced in these tissues afte[r]
transcriptional activation of E2F [35]. Therefore,IxoA-induced pRb inhibition of E2F-1 and DP-1 mayaccount for the accumulation of cells at the G2 ⁄ Mphase of the cell cycle. We show here that sustainedG2 ⁄ M arrest induced by IxoA may be E2F-<[r]
35. Kobayashi N, Hiraoka N, Yamagami W, Ojima H, Kanai Y, Kosuge T,Nakajima A, Hirohashi S: FOXP3+ regulatory T cells affect thedevelopment and progression of hepatocarcinogenesis. ClinCancer Res 2007, 13:902-911.36. Liu L, Chahroudi A, Silvestri G, Wernett ME, Kaiser WJ, Safrit[r]
human epithelial ovarian cancer (EOC) cells possess anintact autocrine bone morphogenetic protein-4 (BMP4)signalling pathway[2]. BMPs comprise approximately 20* Correspondence: tshephe6@uwo.ca1Department of Pharmacology, Dalhousie University, Sir Charles TupperMedical Building,[r]
We determined differentially expressed genes in HT29 human colon cancercells, both after short treatment with methotrexate (MTX) and after theresistance to MTX had been established. Screening was performed usingAtlas Human Cancer 1.2K cDNA arrays. The analysis was[r]
X in human colon adenocarcinoma neoplastic cells andtissues. A growing body of evidence has shown thatnonsteroidal anti-inflammatory drugs that inhibit COX-2can suppress colorectal tumorigenesis [42–45] and thatPGE2, a major COX-2 product, is involved[r]
expression. PBMCs from AM D patients and controlswere treated with or without C5a and a C5aR antagonistfor 3 days. Cell supernatants from 14 controls and 14AMD patients were used for ELISA analysis and are pre-sented side by side in Figure 1A. The add[r]
doi:10.1111/j.1742-4658.2007.06209.xUbiquitination and proteasomal degradation have recently emerged as anadditional level of regulation of activated forms of Rho GTPases. To char-acterize this novel regulatory pathway and to gain insight into its biological[r]
expression. PBMCs from AM D patients and controlswere treated with or without C5a and a C5aR antagonistfor 3 days. Cell supernatants from 14 controls and 14AMD patients were used for ELISA analysis and are pre-sented side by side in Figure 1A. The add[r]
(2000) Murine B cell d ifferentiation is a ccompanied b y pro-grammed expression of multiple novel b-galactoside a2,6-sialyl-transferase mRNA f orm s. Glycobiology 10, 67–75.30. Dalziel, M., Huang, R.Y., Dall’Olio, F., Morris, J.R., Taylor-Papadimitriou, J. & L au, J.T. (2001) M[r]
factor Tiam1. J. Biol. Chem. 278, 400–406.1564 I. D. Jung et al. (Eur. J. Biochem. 271) Ó FEBS 200435. Matsumoto, Y., Tanaka, K., Harimaya, K., Nakatani, F., Mat-suda, S. & Iwamoto, Y. (2001) Small GTP-binding protein, Rho,both increased and decreased cellular motility, activation [r]
FTDP-17 Missense mutations in Tau produce Tau aggregates andother pahotlogical phenotypes in transfected CHO cells. Mol. Biol.Cell. 11, 4093–4014.39. Kosik, K .S. (1992) Alzheimer’s disease: a cell biological perspec-tive. Science 256 , 780–783.40. Alonso, A., Zaidi, T.,[r]
described in Experimental p ro cedure s. E ach bar represents the meanvalue ± SD of cell number as percentage of attenuance in controlcultures.Ó FEBS 2004 Cytotoxicity of RNase T1 via HVJ envelope vector (Eur. J. Biochem. 271) 3571cleaves 28S rRNA at a specific site[r]
and incubated for 2 h at room temperature with rabbit poly-clonal PRDX5 antibody (1 : 2000 dilution) [11] or commer-cial antibodies to actin and NRF2 (1 : 1000 dilution) inTBST containing 5% BSA. The membrane was washed for15 min with TBST and incubated with[r]
One major question that remains unanswered is the effectof receptor ligands on the phenomenon of oligomerization.For different GPCRs, ligands have been reported toincrease, decrease or have no effect on the oligomerizationprocess, which for many GPCRs, seems to be constitutive[1,2]. These app[r]
CN3D software, version 4.1 (NationalCentre for Biotechnology Information, Bethesda, MD, USA). (B) Expression and catalytic activities of Cb1, Cb1D4, Cb3ab and Cb3abD4. Cellextracts of 239T cells, either mock transfected or transfected with expression[r]