PRODUCTION OF KNOCKOUT MICE BY DNA MICROINJECTION OF VARIOUS CRISPR/CAS9 VECTORS INTO FREEZE-THAWED FERTILIZED OOCYTES

and proteomics. Functional genomics addresses g lobal issues of g ene expression, such as lookin g at all the g enes that are activated dur- in g major metabolic shifts (as from g rowth under aerobic to g rowth under anaerobic conditions) or durin g embryo g enesis and development [r]

The above studies indicate that the success of gene therapy depends on the efficiency of gene delivery, and viruses have evolved to become superior for transducing cells under in vivo conditions. The most commonly used viruses in gene therapy are adenoviruses, which are non- integrati[r]

• RBCEVs also deliver Cas9 mRNA and gRNA for genome editing .
• ASOs and CRISPR-Cas9 can be designed and programmed to target any gene of interest , including undruggable targets
• RBCEV-delivery system is suitable for clinical applications because: • RBCs[r]

Recombinant gene delivery vectors derived from Tag -deleted SV40 viruses (rSV40s) are potentially useful tools for transduction of many cell types, both in culture and in animals. Characteristically, these vectors: (i) can be made to very high titers (>10 12 infectious units/[r]

Results
DCs from lupus-prone mice accumulate with age
We studied DCs in spleens of lupus-prone NZM2410 mice in comparison with non-autoimmune age-matched BALB/c and B6 mice. NZM2410 mice, derived by selective inbreeding of the progeny of NZ[r]

Sperm chromatin structure assay (SCSA)
Chromatin in a spermatozoon is condensed to one-sixth of the volume in somatic cells. Nor- mal sperm development leads to a chromatin structure in which the DNA in situ is resistant to denaturation, whereas the DNA of spermatozoa with an abnorma[r]

Since increased expression of proinflammatory chemok- ines including IL-8, in response to various stimuli includ- ing adenovirus vectors can be upregulated by NF-κB transcription factor [22], we employed luciferase reporter assay to examine the inhibition of transc[r]

A recent microarray study [119] revealed that there was only a moderate alteration of gene expression in moderately ADAM10 overexpressing adult mice. Genes coding for pro-inflammatory or pro-apoptotic proteins were not over-represented among differentially regulated genes and, indeed, a[r]

Issued by Date of release Version Page
00 3/5
Derivative from a standard product
Under it we understand the co-ordinating / Matches of a colour, to a surface, a gloss degree, etc.. - It is therefore no development in the sense of a new development and is ther[r]

When data are input using read.table(), or when the data.frame() function is used to create data frames, vectors of character strings are by default turned into factors.. The parameter s[r]

To evaluate the safety of recombinant adenovirus coding the human IL-12 protein in mice. Subjects and methods: BALB/c mice were divided into 3 groups, each group of 9 mice, including control group, recombinant adenovirus coding the human IL-12 protein injection group at 106 vp/head, recombinant aden[r]

Thirdly, we assessed whether the loss of PKCδ protects taste progenitors from death and/or cell cycle arrest by analyzing the proliferation index of taste epithelia in knockout mice at d[r]

contributory except for an intermittent low-grade fever and occasional spikes of a high-grade fever with chills. On examination, he was disoriented and had multiple circu- lar full-thickness burns to the anterior abdomen ranging in size from 2 to 5 cm across with central sparing of t[r]

Relatively large quantities of water are required to produce sugarcane. Because it takes 12 kg of sugarcane to produce 1 L of ethanol, about 7,000 L of water are needed to produce the required 12 kg of sugarcane per liter of ethanol.
Although the Brazilian[r]

Syngenta Seeds’ Gary Wietgrefe points out several of the impediments to widespread adoption of HTF corn (Ed Zdrojewski in BioFuels Journal , 2003b). To begin with, starch and ethanol yield vary by geographic region and from year to year, making an optimizing hybrid choice diffi[r]

IVF is a safe and effective treatment. Between 20 and 30 percent of the fertilized eggs eventually develop into live-born children and the majority of all infertile women treated with IVF succeed in having a child. The risk of complicati[r]

HCM City Univ. of Technology, Faculty of Mechanical Engineering Nguyen Tan Tien
§2. Free-Body Diagram
- A Free-Body Diagram : a sketch of the isolated or free body
which shows all the pertinent weight forces, the externally applied loads, and the[r]

TRANG 12 L IST OF T ABLES Table 2.1 Comparisons of Nγ,circular proposed by various researchers Table 2.2 Summary of projected angle, αp by various researchers modified from Craig and Chu[r]

Objective: To investigate effects of Wullughbeia cochinchinensis to ameliorate disorders in anxiety-like behaviors of mice. Subjects and methods: In the present study, 50 Swiss mice were induced anxiety-like behaviors by scopolamine.

Biotechnology has played an important role towards the attainment of production sustainability of crops by using various biotechnological tools. Biotechnology has the potential to provide new opportunities for achieving enhanced crop productivity in a way that will alleviate poverty, improve food se[r]