CIRCADIAN CLOCK REGULATION THROUGH PRE-MRNA SPLICING: A REVIEW

Kinji Ohno and Akio Masuda
Abstract RNA is not a simple intermediate linking DNA and protein. RNA is
widely transcribed from a variety of genomic regions, and extensive studies on the functional roles and regulations of noncoding RNAs including antisense RNAs and small RNAs are i[r]

Results and Discussion
In vitro self-splicing of DiGIR2 and DiGIR2 D P9.2 ribozymes from heterologous transcripts
To test the potential of DiGIR2 and DiGIR2 D P9.2 in RNA reprogramming, the ribozymes were first inserted in cis into heterologous exons that represent therapeutic, relevant[r]

Furthermore, alterations in AM function during sepsis-induced hypoxia may influence TNF secretion and the progression of acute lung injury. It was proposed that acute changes in partial pressure of oxygen ( p O 2 ) tension surrounding AMs alter NF- κ B activation and TNF secretion in these lung cell[r]

TRANG 5 Decreased Ago2 expression was associated with attenuated miRNA-mRNA interaction under starvation condition In order to determine the mechanisms through which miRNA-mRNA interacti[r]

Exons and Coding
What’s the difference between exons and coding sequence?
Exons often are described as short segments of protein coding sequence. This is a bit of an oversimplification. Exons are those segments of sequence that are spliced together after the introns have been removed[r]

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Mainly vegetables
You like the simple life, nothing too strenuous. A regular 9-5 job would suit you down to the ground. You don't really like planning ahead, you play it by ear and go with the flow. You take things one day at a time, a[r]

Real time PCR was used for determination of levels of sPLA 2 -IIA mRNA in brain tissues. Taqman primers and probes specific for human sPLA 2 -IIA and ribosomal 18S RNA were obtained from Applied Biosystems (Foster City, CA). For each sample (analyzed in triplicate), a pool con- taini[r]

3.2. Application to tandem repeats
Here we further explore the utility of the mutual informa- tion statistic, and we examine its performance on the prob- lem of detecting short tandem repeats (STRs) in genomic se- quences. STRs, usually found in noncoding regions, are made of back-to-back repeti[r]

3.2. Application to tandem repeats
Here we further explore the utility of the mutual informa- tion statistic, and we examine its performance on the prob- lem of detecting short tandem repeats (STRs) in genomic se- quences. STRs, usually found in noncoding regions, are made of back-to-back repeti[r]

1
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium; 2 Department of Biochemistry, School of Medical Sciences, University of Bristol, UK
In a search for genes regulated in response to cAMP we have identified a new protein, p76 RBE , wh[r]

or circadian fluctuations in specific immune variables should be performed in order to establish the possible implications of Gal1 in the mammalian circadian system. To illustrate this concept, Gal1-mediated tolerogenic mechanisms, including promotion of T cell apoptosis, modulation of[r]

3-kinase (PtdIns 3-kinase) that acts either downstream or upstream of the MAP kinases.
To determine the role of these signaling molecules in CTGF / CCN2 expression, cells were pretreated for 30 min with Pd-098059 (20 l M ), SB-20856 (10 l M ), SP-600125 (10 l M ), or worthmanin (10 l M ), which[r]

To examine whether the hypoxic repression of Dicer pro-tein levels was regulated through repression of Dicer mRNA levels, we examined Dicer mRNA levels by RT PCR in MCF7 cells exposed to[r]

accreditation when making decisions about private giving.
 Easing transfer. Accreditation is important to students for smooth
transfer of courses and programs among colleges, universities and programs. Receiving institutions take note of whether or not the credits a student wishes to t[r]

Plants constantly monitor changes in photoperiod or day length to trigger the flowering cycle at the most appropriate time of the year. It is well established that photoperiodic flowering is intimately associated with the circadian clock in Arabidopsis. In support of this notion, many clock-defectiv[r]

In this context, we quantified molecular parameters focusing on the BRCA1 gene expression regulation and function BRCA1 promoter methylation, BRCA1 in situ mRNA expression, BRCA1in situp[r]

showing that expression of the PAC1 receptor gene was not significantly changed in STZ-induced diabetic rats. In apoptotic cascades, Bcl-2 family members are major regulators of mitochondrial integrity and mito- chondrion-initiated caspase activation [34]. The Bcl-2 protein family consists of pro-apo[r]

RGS1 and RGS2 are proven to be the main RGS mRNA modulated in murine macrophages after stimu- lation with LP, LPS, poly(I:C) and ODN1826. Micro- array analysis identified RGS2 mRNA as the most downregulated gene after 6 h of stimulation (Table 1), whereas interleukin-6 was the strongest[r]

The co-occurring pair hypothesis predicts that muta- tions that occur in these motifs should have a higher likelihood of disrupting exon splicing than those that occur in the same motifs when they are alone. There- fore, the former would be more likely to be eliminated by purifying sel[r]

The circadian clock and cell cycle are two global regulatory systems that have pervasive behavioral and physiological effects on eukaryotic cells, and both play a role in cancer development. Recent studies have indicated that the circadian and cell cycle regulator, TIMELESS, may serve as a molecular[r]