BÁO CÁO Y HỌC: "WOUND HEALING AND INFLAMMATION GENES REVEALED BY ARRAY ANALYSIS OF ''''MACROPHAGELESS'''' PU.1 NULL MICE" PPT
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relatively similar pattern to that found in the untreated control cells. The pairwise comparison analysis demonstrated four significantly up-regulated (COBRA1, ITGB4, STAU2, and HMGN3) genes and one down-regulated (ANK3) gene. All these genes exert their function on transcriptional and transl[r]
118mouse embryonic stem (ES) cells carrying targeted mutations of BRCA1 (homozygous deletion of exons 22-24, referred to as BRCA1-/-) or BRCA2 (trEx11/ΔEx27, referred to as BRCA2-/-), Farmer et al. (2005) revealed similar sensitivity of these cells to PARP-1 inhibitors in vitro [25]. Will thi[r]
date the mechanism of this network. However, a poor correlation between mRNA and protein abundance has also been reported [9]. Furthermore, a single gene can encode for more than one mRNA species through differential splicing, and proteins can undergo as many as 200 posttranslational modifications.[r]
Germany). HDL was measured following precipitation of apoB containing lipoproteins with phosphotungstate (Boehringer Mannheim). Apoliprotein AI (Apo AI) and Apo B were measured using the Beckmann Array 360 (Beckmann Instruments). Genotyping DNA was extracted from white blood cells using standard met[r]
yk23724@gipac.shinshu-u.ac.jp Received: 2006.03.25; Accepted: 2006.06.30; Published: 2006.07.12 Background: Helicobacter pylori has now been widely recognized as a causative agent of gastroduodenal diseases. The development of safer anti- H. pylori compounds is desirable due to the antibiotic-resist[r]
from MedTex Company, Istanbul, Turkey. The chromatogram was developed with sodium phosphate and sodium azide buffers. Abnormal Hb was 20.0 % of the total Hb; HbA2; 2.0% and Hb A ;77.3% [6]. HbF value was determined by alkali denaturation method and found as 0.7 % [7]. The p50 values obtained (using[r]
and peripheral blood (PB) samples from 13 Patients with AML were obtained at the time of their initial diagnosis after informed consent. For control, 51 PB samples were obtained from voluntary healthy individuals after informed consent. Mononuclear cells were separated by density gradient centrifuga[r]
with compensated cirrhosis due to hepatitis C has been evaluated in several trials, both as the focus of prospective study, as well as in subgroup (post hoc) analyses of large registration trials. Despite the improved efficacy of peginterferons, the sustained virological response (SVR) rate is subop[r]
1/10 to 1/100 dilution in order to achieve accurate quantification. The most promising approach for the future is fully automated real-time PCR assays, which are faster, more sensitive than classical target amplification techniques and are not prone to carryover contamination. Molecula[r]
the MTT assay. The results showed that hESC survivability after exposure to 25oC and 4oC for 24h was 77.3 ± 4.8 % and 64.4 ± 4.4 % respectively (significantly different, P < 0.05). The corresponding survival rates after 48h exposure to 25oC and 4oC was 71.0 ± 0.5 % and 69.0 ± 2.3 % respective[r]
Received: 2006.07.07; Accepted: 2006.10.25; Published: 2006.10.27 Aim: To investigate the association between meat intake and incidence of type 2 diabetes (type 2 DM) in a large cohort of middle-aged women. Design, subjects and methods: Incident cases of type 2 DM were identified during an average o[r]
by all nucleated cells and is secreted into the blood at a constant rate [1, 2]. Cys-C is freely filtered through the normal glomerular membrane and completely reabsorbed, followed by catabolization by the proximal tubular cells [1, 2]. The biological fates of Cys-C are favorable as an[r]
protein as a repressor of HBV production. They found a moderate 2-4 fold reduction of HBV mRNA and HBV antigen expression in the presence of HCV structure genes and a stronger up to 20 fold suppression of HBV particle secretion. Furthermore, the target structure of HCV core protein was identified in[r]
by a washed step and incubated with ethanol (1 mM) for 24 h. Then, ethanol-induced apoptosis was analyzed by a double-fluorescence staining technique with Hoechst 33342 (excitation 330-380 nm, emission 460 nm; Molecular Probes, MoBiTec, Göttingen, Germany) and propidium iodide (excitation 590[r]
As an example we presented the finding that maternal use of loratadine in early pregnancy was associated with a roughly doubled risk for infant hypospadias. We concluded: “The finding can still be random, but causality cannot be dismissed, even though the mechanism of action is not understood”. A re[r]
glycemic control. Strength training is the most effective lifestyle intervention to increase muscle mass but limited data is available in older adults with diabetes. We determined the influence of strength training on muscle quality (strength per unit of muscle mass), skeletal muscle fiber hypertrop[r]
Mammography and ultrasound revealed well circumscribed mass in the lower outer quadrant of the right breast. The excisional biopsy was performed. Malignant phyllodes tumor has been diagnosed. The tumor was completely excised. Clinical follow-up for 12 months has not revealed any evidence of focal di[r]
of general populations [8, 9], and as high as 30% of the population aged 65 and older in the Framingham Heart Study (tHcy > 14 nmol/ml) [10]. Increasing tHcy concentrations accelerate cardiovascular diseases by promoting vascular inflammation, endothelial dys-function, and hypercoagulability[r]