BÁO CÁO KHOA HỌC: "CHARACTERIZATION OF HC58CDNA, A PUTATIVE CYSTEINE PROTEASE FROM THE PARASITE HAEMONCHUS CONTORTUS" PPS

-Tyrosine 0.57 0.194 0.453 0.79 0.35 8.74 18.6 53 67D-Tyrosine 0.32 0.216 0.505 1.6 0.24 7.98 17.0 71 44Tyramine 0.32 1.59 3.72 12 0.12 27.6 58.7 490 41Ó FEBS 2003 Tyrosinase from the mollusk, Illex argentinus (Eur. J. Biochem. 270) 4033Furthermore, the nucleotide sequences of cDNA-1 andcDNA-[r]

single papain in papaya [18]. Another cysteine protein-ase, CC-23, was purified from V. cundinamarcensislatex and its corresponding DNA fragment cloned byPereira et al. [20]. The N-terminal sequence appearedto be different from the N-terminal sequences reportedfor CC-I to CC-IV. The authors suggested[r]

from five cell lines by using the Qiashredder/RNeasy Kit (Qiagen) and5 lg of RNA was loaded into each lane of a denaturing formaldehydeagarose gel. Following electrophoresis, the RNA was transferred to anitrocellulose membrane and probed with a32P-labeled cDNA probeconstructed using the[r]

´e, V., Froger, A., Deschamps, S., Pellerin, I., Delamarche,C., Bonnec, G., Gouranton, J., Thomas, D. & Hubert, J.F. (1998)Oligomerization state of water channels and glycerol facilitators.Involvement of loop E. J. Biol. Chem. 273, 33949–33953.28. Verbavatz, J.M., van Hoek, A.N[r]

similar regulatory mechanisms in these specific tissues.The coexistence in the same organism of multiple tryptasegenes, as found here, is in linewith findings reported by othersfor human [23–26], and mouse [27,28] tryptases. What ispeculiar here is the presence in the same organism ofisoforms, BLCT an[r]

Đình Thi, Nông Văn Hải (2010), “Biểu hiện yếu tố hoạt hóa plasminogen mô (h-tPA) người ở tế bào”, Kỷ yếu hội nghị sinh học phân và hóa sinh y học, tr. 185-191. 14 12. Nguyễn Nam Long, Nguyễn Bích Nhi, Phan Văn Chi (2005), “Tách dòng gen mã hóa interleukin-2 của người”, Y học Việt Nam 310(5), tr. 26[r]

ences are explanatory of different aromas of fruits.Here we describe the cloning and characterization ofan AAT expressed in the fruit of Royal Gala andreport on the kinetic characterization of the enzyme.ResultsThe MpAAT1 gene and its predicted proteinGene mining identified 20 acyl transferase-encodi[r]

nase). On the basis of sequence homology, GHF 5 enzymescan be further divided into five subfamilies [1,25]. P. hilariscellulase is closely related to subfamily 2 members, which iscomposed only of cellulases from bacteria, fungi andnematodes. It has been shown that the nematode cellulasegenes contain[r]

An RNA depurination test was used to confirm thein vitro enzymatic activity of rPAC. Figure 3 shows anFig. 1. Deduced amino acid sequence of recombinant pulchellin A-chain (rPAC) aligned to abrin-a, abrin-c and ricin (RTA) A-chains. Conservedamino acids are highlighted in gray. r[r]

plant cell suspension cultures of taxonomically distantspecies tested to date, virtually all exhibited MJE activity[20]. O ne might s peculate that the M JEs of different speciesare encoded by orthologous genes.Whenever analysed, plant cells and tissues contain JAand its methyl ester, MeJA, side by[r]

ed at the serine and threonine residues upon cellular activation. We haveshown that Ki-1 ⁄ 57 interacts with chromo-helicase DNA-binding domainprotein 3 and with the adaptor ⁄ signaling protein receptor of activatedkinase 1 in the nucleus. Among the identified proteins that interacted withKi-1 ⁄ 57 i[r]

interact with bound peptide–substrate, such as Arg240,Asn316 and Tyr318 in rat PHM [7,41] were also con-served in planarian PHM (marked with ! in Fig. 4).Percentage amino acid identities between these repre-sentative PHMs and PAMs were calculated for theirPHM core regions and then tabulated (Table 1[r]

the effects of phalloidine [1,2]. Another toxic polypep-tide, phallolysin, possessing hemolytic activity, wasalso detected in the fruit body of the death cap [3–7].Its chemical properties and biological activity, as wellas its mechanisms of action, have been described previ-ously [2,8].Many mushroom[r]

virus X protein is both a substrate and potential inhibitor ofthe proteasome complex. J Virol 1999, 73:7231-7240.5. Huang J, Kwong J, Sun EC, Liang TJ: Proteasome complex as apotential cellular target of HBX protein. J Virol 1996,70:5582-5591.6. Zhang Z, Torii N, Furusaka A, Malayaman[r]

virus X protein is both a substrate and potential inhibitor ofthe proteasome complex. J Virol 1999, 73:7231-7240.5. Huang J, Kwong J, Sun EC, Liang TJ: Proteasome complex as apotential cellular target of HBX protein. J Virol 1996,70:5582-5591.6. Zhang Z, Torii N, Furusaka A, Malayaman[r]

generated from the one and same gene, and thereby alsocontrol the biological effects elicited by the interaction ofthese two isoforms. Alternatively, it could be speculated thatthe secreted isoform of MUC15 may function as a protectivemucin, perhaps as a coconstituent with gel-forming[r]

Acknowledgements The H1N1— A/WSN/1933 vRNP expression plasmids were kindly provide by Prof. George Brownlee (Sir William Dunn School of Pathology, University of Oxford). The pandemic H1N1 viral RNA (A/Auckland/1/2009) was kindly provided by Ian G Barr, World Health Organization Collabo[r]

Acknowledgements The H1N1— A/WSN/1933 vRNP expression plasmids were kindly provide by Prof. George Brownlee (Sir William Dunn School of Pathology, University of Oxford). The pandemic H1N1 viral RNA (A/Auckland/1/2009) was kindly provided by Ian G Barr, World Health Organization Collabo[r]

using boiled cell-free extract; E: using cell-free extract prepared from the transformant with no induction of the GAL1 promoter; F: using cell-free extract prepared from the transformant with void vector. MS fragmentations of entries A and B are shown in the lower panel.M. Shibuya et al. CYP[r]

FL (439 bp)ΔΔE18 (387 bp)1234 56 78910111213500400300(bp)ABFig. 1. Gene structure and expression of a novel STAT5A splicing variant. (A) The mouse STAT5A gene contains 20 exons. The translationinitiation codon and stop codon are located in exons 3 and 20, respectively. ST[r]