INTRACELLULAR DELIVERY OF MESSENGER RNA BY RECOMBINANT PP7 VIRUS-LIKE PARTICLES CARRYING LOW MOLECULAR WEIGHT PROTAMINE

The chromatin of the sperm of M. surmuletus is organized in bundles of parallel DNA molecules Mullus surmuletus sperm chromatin was digested with micrococcal nuclease and treated afterwards as des- cribed in Experimental procedures to obtain fractions SI, SII and PII. Figure 4 s[r]

Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) is one of the most widespread pathogens of Indian non-mulberry silkworm, A. mylitta . CPV-infected
A. myllita larvae develop chronic diarrhea that even- tually leads to a condition known as “ Grasserie ” and ultimate dea[r]

mation of particles that comigrated with VLPs purified from E. coli . Thus, although assembly requires RNA, it does not depend specifically on PP7 RNA. However, when assembly was conducted at high concentrations of PP7 operator RNA an additional electrophoretic species was fo[r]

Mutations that are identified in the viral genome can the- oretically be introduced at any of the various steps that lead to proviral DNA either during the synthesis of the minus-strand cDNA copy of the genomic RNA template, during the synthesis of the second DNA strand w[r]

and nucleotide sequence analysis), suggesting the com- plexity was not necessarily due to genomic rearrange- ments upon virus regeneration and an additional passage in Vero cells (Fig. 5A, lanes 1–4). The presence of the 1.0 kb amplicon, which is suggestive of EGFP gene deleti[r]

which cannot be metabolized by the body and the dimer may remain in the body for 6 months [34] . RA ( cis - 12 - hydroxyoctadeca - 9 - eonoic acid) was found to be the most appropriate alternative for the synthesis of the fatty acid - based polyanhy- drides. It is one o[r]

Xem Thêm " SYNTHESIS OF HYPERBRANCHED POLYMERS VIA POLYMERIZATION OF ASYMMETRIC MONOMER PAIRS "

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The flower-like CuO materials with a good uniformity were successfully synthesized by the selfassembly method. By using pH ranging from 7 to 9, CuO provided different morphologies. X-ray diffraction (XRD) and Scanning electron microscopy (SEM) revealed the purity and uniformity of the CuO particles,[r]

Squamous cell carcinoma of the oral cavity (OSCC) is a common cancer form with relatively low 5-year survival rates, due partially to late detection and lack of complementary molecular markers as targets for treatment. Molecular profiling of head and neck cancer has revealed biological similarities[r]

Results
Design and synthesis of peptides mimicking extracellular loops of the human TP receptor
Our approach for the structural characterization of the TP extracellular loops is the use of synthetic peptides mimicking the loop domains. Analysis of the TP recept[r]

The technology of recombinant single chain variable fragments (scFvs) expression has been used in research, diagnosis and treatment of diseases. In the previous study, we studied the expression of a recombinant single chain variable fragment recognizing blood A antigen (antiA-scFv) in E. coli. Howev[r]

from the bottom exit region F through the left side. This requires to look ahead into the region J. Let us consider the rightmost green particle. If we number the upper left side of region J from bottom to top, then its endpoint is precisely k + µ1 (the length of the first row of<[r]

The nosology of OI has been a subject of discussion and revision in light of recent discoveries of novel genes causing recessive forms of the disease. Plotkin (Plotkin, 2004) proposed to classify as OI all those cases in which a mutation in either COL1A1 or[r]

meeting abstracts
DVT = deep vein thrombosis; ICU = intensive care unit; LMWH = low-molecular-weight heparin; VTE = venous thromboembolism.
Available online http://ccforum.com/content/5/6/277
Deep vein thrombosis (DVT) remains an underestimated problem in ICU patients, des[r]

mum ethanol concentration (18 g/l) within the short time (36 h) of the recombinant yeast strains.
A comparison of conventional high-temperature and low-temperature cooking fermentation systems using the yeast strain YF207/pGA11/pAA12 co-displaying glucoamylase[r]

Wheat (AABBDD, 2n = 6x = 42) is a major dietary component for many populations across the world. Bread-making quality of wheat is mainly determined by glutenin subunits, but it remains challenging to elucidate the composition and variation of low-molecular-weight glutenin subunits (LMW-GS) genes, th[r]

Results
leading to clathrin-mediated endocytosis of virus particles (Chu & Ng, 2004). As such, a clustering of the FERM domain of RADIXIN, FRMD4A, TALIN and FAK was performed. It revealed two identical amino acids and a num[r]

With raw materials to the easier discoverableness on the bundles or the pallet by means of spraying colour, marker the internal raw material designation one applies.
Store
the goods are properly stored, i.e. according to their relevant request. It is to be made certain that b[r]

20 2 POLYMERISATION 31 2.1 Polymerisation mechanisms 31 2.2 Polymerisation processes 36 2.3 Polymerisation reactors 39 2.4 Catalysts 42 2.5 Molecular weight and molecular weight distribu[r]

Annexin V-FITC showed enhanced apoptosis by VEVDMs
To measure and compare the extent of apoptosis induced by 1 μ M of free DOX and VEVDMs on incuba- tion for 24 h, FITC-conjugated annexin staining was done and analyzed using flow cytometer. Annexin stain- ing which iden[r]

Synovitis in osteoarthritis (OA) the consequence of low grade inflammatory process caused by cartilage breakdown products that stimulated the production of pro-inflammatory mediators by fibroblast-like synoviocytes (FLS). FLS participate in joint homeostasis and low grade inflammation in the joint m[r]