BÁO CÁO Y HỌC: " STATUS OF COMPLETE PROTEOME ANALYSIS BY MASS SPECTROMETRY: SILAC LABELED YEAST AS A MODEL SYSTEM" PPT

Received: 2006.07.07; Accepted: 2006.10.25; Published: 2006.10.27 Aim: To investigate the association between meat intake and incidence of type 2 diabetes (type 2 DM) in a large cohort of middle-aged women. Design, subjects and methods: Incident cases of type 2 DM were identified during an av[r]

“Ex-smokers” were identified as those who had stopped smoking >1 year before the 1968-1969 examination. Anthropometric measurements in 1968-1969; Body height and body weight were measured with the subjects wearing only briefs. Body mass index (BMI) was calculated by dividing body weight in kg[r]

replicating HCV RNA. Critical for the usefulness of this system was the identification of specific amino acid substitutions, i.e., cell culture-adaptive changes, that increased the efficiency of replication initiation by several orders of magnitude [9]. With the replicon system it became possible, f[r]

. The PCR was con-ducted with initial denaturation at 95°C for 10 minutes, 30 cycles of denaturation at 95°C for 1 minute, an-nealing at 60°C for 1 minute, and extension at 72°C for 1 minute, and a final extension at 72°C for 5 minutes. The primers were F1: 5’-AGC CTC TCC TGA CTG TCA TCC-3’,[r]

by all nucleated cells and is secreted into the blood at a constant rate [1, 2]. Cys-C is freely filtered through the normal glomerular membrane and completely reabsorbed, followed by catabolization by the proximal tubular cells [1, 2]. The biological fates of Cys-C are favorable as an endoge[r]

glycemic control. Strength training is the most effective lifestyle intervention to increase muscle mass but limited data is available in older adults with diabetes. We determined the influence of strength training on muscle quality (strength per unit of muscle mass), skeletal muscle fiber hypertrop[r]

were measured at the beginning and end of the 30-day treatment phase. BMI was calculated as the body weight in kilograms divided by the square of the height in meters. Bodv Weight and Composition Body weights, performed on undressed indi-viduals, were measured using a calibrated Bodymas-ter S[r]

. The cells were grown to 80% confluence. After 2 days of cell culture, the cells were harvested with 0.05% trypsin / 0.02% EDTA (Gibco) and seeded in 6-well plates (Falcon) at concentrations of 1x105/ml. Six sets of experiments were performed. Each set consist of two groups as follow: group 1, HepG[r]

Germany). HDL was measured following precipitation of apoB containing lipoproteins with phosphotungstate (Boehringer Mannheim). Apoliprotein AI (Apo AI) and Apo B were measured using the Beckmann Array 360 (Beckmann Instruments). Genotyping DNA was extracted from white blood cells using standard met[r]

AAG GTG GAG ATC ATC GC-3’. PTEN: sense, 5'-CCA ATG TTC AGT GGC GGA ACT-3; antisense, 5'-GAA CTT GTC TTC CCG TCG TGTG-3'. GAPDH: sense, 5’-CAT GGA GAA GGC TGG GGC TC-3’, an-tisense, 5’-CAC TGA CAC GTT GGC AGT GG-3’. The conditions used for the PCR were as follows: 94°C for 2 min, 30 cycles of 94°C fo[r]

A group of 16 females, aged 80-93 years old, was divided into 2 groups according to their baseline DHEA-S lev-els: Lower Halves (N = 8) and Upper Halves (N = 8), and participated in a 4-month exercise intervention trial. Insulin response with an oral glucose tolerance test (OGTT), chol[r]

The controls were selected from the study population of 65,383 male births. The control group comprised of 10 controls per case, and these controls had no recorded diagnosis of hypospadias during the first six months post delivery. We matched on birth, month, and year of the child. To examine whethe[r]

compared to those with ND. These results are consistent with a previous report among HAART-experienced subjects, thus further implicating HIV DNA in the pathogenesis of HAD. Key words: human immunodeficiency virus type 1; dementia; cognition; HIV DNA 1. INTRODUCTION Complete eradication of th[r]

immunosuppression on recurrent HCV [1,4]. However, this is extremely difficult to achieve in most patients. In the post-liver transplant setting the differential diagnosis of elevated liver chemistry tests is broad and includes acute cellular or chronic ductopenic rejection, bacterial or viral (CMV[r]

QuantiTect SYBR green RT-PCR kit (Qiagen, Hilden, Germany). Specific primers for transcription factor (AP1, SRF, Elk1, Stat1 and NFκB) and members of the Fas receptor signaling pathway (Fas receptor, Fas Int. J. Med. Sci. 2006, 3 162ligand, FADD and caspase 8) were used. Primer design was performed[r]

the intracellular pool to the plasma membrane [5, 6]. However, sustained insulin deficiency leads to a decreased number of GLUT4 transporters, resulting in impaired responsiveness of glucose transport to both insulin and exercise [4, 7]. People with type 2 diabetes have been shown to have def[r]

Int. J. Med. Sci. 2006, 3 148International Journal of Medical Sciences ISSN 1449-1907 www.medsci.org 2006 3(4):148-151 ©2006 Ivyspring International Publisher. All rights reserved Short Research Communication Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphism Dani[r]

immunosuppression on recurrent HCV [1,4]. However, this is extremely difficult to achieve in most patients. In the post-liver transplant setting the differential diagnosis of elevated liver chemistry tests is broad and includes acute cellular or chronic ductopenic rejection, bacterial or viral (CMV[r]

Key words: phyllodes tumor, liposarcoma, tubular adenoma 1. Introduction Phyllodes tumors are rare entity in the breast. They make up less than 1% of all breast tumors [1]. The majority of these lesions behave in a completely benign fashion in that they do not have metastatic potential, leavi[r]

Int. J. Med. Sci. 2006, 3 117International Journal of Medical Sciences ISSN 1449-1907 www.medsci.org 2006 3(4):117-123 ©2006 Ivyspring International Publisher. All rights reserved Review PARP-1 inhibitors: are they the long-sought genetically specific drugs for BRCA1/2-associated breast cancers? Jos[r]